Ho Chi Minh City University of Technology, Vietnam National University-Ho Chi Minh City, Ho Chi Minh City, 703500, Vietnam.
Nguyen Tat Thanh University, Ho Chi Minh City, 702000, Vietnam.
J Nanosci Nanotechnol. 2019 Feb 1;19(2):974-978. doi: 10.1166/jnn.2019.15925.
High concentration of rutin nanosuspension (5%) in water was prepared by using high-speed and high-pressure homogenizer. Sodium tripolyphosphate (STPP) crosslinker and chitosan polymer were found to be an efficient encapsulating reagent and stabilizer to prepare rutin nanosuspension. In addition, the particle sizes and sedimentation rates of rutin nanosuspension were investigated under effects of several factors, including applied pressures of homogenization, the presence of co-solvent PEG 400 (polyethylene glycol 400), chitosan loading, STPP and chitosan mass ratio. Raw rutin material was characterized using powder X-ray diffraction (XRD) and high-performance liquid chromatography (HPLC). To evaluate the parameter of rutin nanosuspension, laser diffraction spectrometry (LDS) was employed to estimate the particle size distribution. Rutin nanosuspension prepared under optimal conditions was freezing dried/spray dried to form rutin powders. Properties of the rutin powders were evaluated using XRD analysis and re-dispersion into aqueous solutions of different pH values.
采用高速高压匀浆法制备了浓度为 5%的芦丁纳米混悬剂。三聚磷酸钠（STPP）交联剂和壳聚糖聚合物被发现是一种有效的包封试剂和稳定剂，可用于制备芦丁纳米混悬剂。此外，研究了在几种因素的影响下，包括匀浆的应用压力、助溶剂聚乙二醇 400（PEG 400）的存在、壳聚糖负载量、STPP 和壳聚糖的质量比，对芦丁纳米混悬剂的粒径和沉降率的影响。采用粉末 X 射线衍射（XRD）和高效液相色谱（HPLC）对芦丁原料进行了表征。为了评估芦丁纳米混悬剂的参数，采用激光衍射光谱法（LDS）估计粒径分布。在最佳条件下制备的芦丁纳米混悬剂经冷冻干燥/喷雾干燥形成芦丁粉末。通过 XRD 分析和在不同 pH 值的水溶液中的再分散性评估芦丁粉末的性质。
