Delforge Marie-Luce, Eykmans Joëlle, Steensels Deborah, Costa Elena, Donner Catherine, Montesinos Isabel
Department of Microbiology, National Reference Center for Congenital Infections, CUB-Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
Department of Microbiology, National Reference Center for Congenital Infections, CUB-Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
Diagn Microbiol Infect Dis. 2019 Mar;93(3):208-212. doi: 10.1016/j.diagmicrobio.2018.09.013. Epub 2018 Oct 4.
CMV IgG avidity assays are widely used and can be helpful in pregnant women to date the onset of CMV primary infection; however, these tests are not standardized and sometimes give inconclusive results. We evaluated the performances of Mikrogen recomLine CMV IgG and IgG Avidity compared to the VIDAS CMV IgG Avidity. On a first sample set of 89 sequential sera collected from 40 women with precisely determined onset of CMV primary infection, the combination of Mikrogen recomLine CMV IgG and IgG Avidity showed an accurate interpretation in 83.1% (74/89), an incorrect result in 4.5% (4/89), and an inconclusive result in 12.4% (11/89) and showed a better sensitivity to diagnose infections <14 weeks compared to VIDAS (85.9% vs. 76.9%). On a second sample set of 89 sera with an intermediate VIDAS CMV IgG Avidity, the combination of line immunoassays provided additional information on the time of infection in 79% (70/89) of the samples. This combination of line assays is useful as additional confirmatory testing and can help to date more precisely the onset of CMV primary infection.
巨细胞病毒（CMV）IgG亲和力检测被广泛应用，对孕妇确定CMV初次感染的发病时间可能有所帮助；然而，这些检测方法并不标准化，有时结果也不明确。我们将Mikrogen重组线免疫分析法检测CMV IgG和IgG亲和力的性能与VIDAS CMV IgG亲和力检测进行了比较。在从40名CMV初次感染发病时间精确确定的女性中收集的89份连续血清的第一组样本中，Mikrogen重组线免疫分析法检测CMV IgG和IgG亲和力的组合在83.1%（74/89）的样本中解读准确，在4.5%（4/89）的样本中结果错误，在12.4%（11/89）的样本中结果不明确，并且与VIDAS相比，在诊断感染时间<14周的情况时表现出更高的敏感性（85.9%对76.9%）。在第二组89份VIDAS CMV IgG亲和力处于中等水平的血清样本中，线免疫分析组合在79%（70/89）的样本中提供了关于感染时间的额外信息。这种线检测组合作为额外的确认性检测很有用，有助于更精确地确定CMV初次感染的发病时间。
