Fredholm B B, Jondal M, Nordstedt C
Biochem Biophys Res Commun. 1987 May 29;145(1):344-9. doi: 10.1016/0006-291x(87)91327-1.
The accumulation of cyclic AMP in Jurkat cells was stimulated by adenosine and adenosine analogues. The accumulation of cyclic AMP induced by these agents was competitively antagonized by the adenosine receptor antagonist 8-p-sulphophenyl-theophylline (KD appr 1.9 microM). The lectin PHA, the diacylglycerol OAG as well as tumor promoting phorbol esters enhanced the accumulation of cyclic AMP induced by the adenosine analogue NECA. The results suggest that activation of CD2/CD3 receptors by lectins could potentiate the endogenous cyclic AMP stimulator adenosine via activation of protein kinase C.
腺苷和腺苷类似物可刺激Jurkat细胞中环磷酸腺苷（cAMP）的积累。这些试剂诱导的cAMP积累被腺苷受体拮抗剂8-对磺基苯基茶碱（KD约为1.9 microM）竞争性拮抗。凝集素PHA、二酰基甘油OAG以及促肿瘤佛波酯可增强腺苷类似物NECA诱导的cAMP积累。结果表明，凝集素激活CD2/CD3受体可通过激活蛋白激酶C增强内源性cAMP刺激剂腺苷的作用。
