Massa G, Wyllie J P, Pratt A M, Molineux G, Schofield R
Br J Haematol. 1987 May;66(1):11-4.
By using karyotypic analysis of female mice treated with busulphan or isopropyl methane sulphonate (IMS), and injected with male bone marrow the donor contribution to both total marrow cellularity and spleen colony forming cells (CFU-S) was assessed for up to 6 months after transplant. In the mice treated with busulphan the marrow cells yielded metaphases of which between 40% and 83% were of donor type. Between 60% and 97% of metaphases in spleen colonies formed in irradiated mice were of donor type during the 24-week study period. In contrast, mice prepared for the transplant with IMS showed no cells of donor type at any time after transplant, neither did they possess CFU-S of donor type. We were therefore led to conclude that the donor cells made no contribution to longterm engraftment in mice prepared with IMS, whilst in those prepared with busulphan they were the predominantly active haemopoietic cells. These results are consistent with a model of haemopoiesis in which the most primitive cells reside in a 'niche' where they are resistant to the effects of IMS but susceptible to the action of busulphan. Busulphan may vacate some niches to allow engraftment by transplanted marrow, whilst IMS yields no unoccupied niches for grafted cells to occupy, and cannot therefore lead to a stable chimaerism.
通过对接受白消安或异丙基甲磺酸酯（IMS）处理并注射雄性骨髓的雌性小鼠进行核型分析，评估移植后长达6个月内供体对总骨髓细胞数量和脾集落形成细胞（CFU-S）的贡献。在用白消安处理的小鼠中，骨髓细胞产生的中期分裂相中，40%至83%为供体类型。在为期24周的研究期间，受照射小鼠脾脏集落中的中期分裂相有60%至97%为供体类型。相比之下，用IMS预处理用于移植的小鼠在移植后的任何时间都未显示出供体类型的细胞，它们也不具有供体类型的CFU-S。因此，我们得出结论，在用IMS预处理的小鼠中，供体细胞对长期植入没有贡献，而在用白消安预处理的小鼠中，它们是主要的活跃造血细胞。这些结果与一种造血模型一致，即最原始的细胞存在于一个“龛位”中，在那里它们对IMS的作用具有抗性，但对白消安的作用敏感。白消安可能会腾出一些龛位，以允许移植的骨髓植入，而IMS不会产生未被占据的龛位供移植细胞占据，因此不能导致稳定的嵌合体形成。
