标准检测方案：基于16S rRNA基因的聚合酶链反应（PCR）和限制性片段长度多态性分析（RFLP）
Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene.
作者信息
Bertaccini Assunta, Paltrinieri Samanta, Contaldo Nicoletta
机构信息
Phytobacteriology Laboratory, DISTAL, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
出版信息
Methods Mol Biol. 2019;1875:83-95. doi: 10.1007/978-1-4939-8837-2_7.
Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.
植原体的检测与鉴定主要基于聚合酶链式反应（PCR），随后进行限制性片段长度多态性分析。该方法可检测并区分植原体，包括那些尚未鉴定的植原体。本方案描述了此方法在扩增子上以及在相同序列的计算机模拟中，用于鉴定16S核糖体RNA（16Sr）组和16Sr亚组水平的植原体的应用。
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