Mehle Nataša, Dreo Tanja
National Institute of Biology, Ljubljana, Slovenia.
Methods Mol Biol. 2019;1875:171-186. doi: 10.1007/978-1-4939-8837-2_14.
Digital PCR-based methods, such as droplet digital PCR, are one of the best tools for determination of absolute nucleic-acid copy numbers. These techniques avoid the need for reference materials with known target concentrations. Compared to real-time PCR, they provide higher accuracy of quantification at low target concentrations, and have higher resilience to inhibitors. In this Chapter, we describe the droplet digital PCR workflow for the detection and quantification of flavescence dorée phytoplasma.
基于数字PCR的方法，如液滴数字PCR，是测定绝对核酸拷贝数的最佳工具之一。这些技术无需使用已知靶标浓度的参考材料。与实时PCR相比，它们在低靶标浓度下提供更高的定量准确性，并且对抑制剂具有更高的耐受性。在本章中，我们描述了用于检测和定量葡萄黄化植原体的液滴数字PCR工作流程。
