Degli Esposti M, Crimi M, Samworth C M, Solaini G, Lenaz G
Biochim Biophys Acta. 1987 Jul 22;892(3):245-52. doi: 10.1016/0005-2728(87)90228-3.
The circular dichroic spectrum of the mitochondrial cytochrome bc1 complex isolated from bovine heart has been resolved into the contributions from the prosthetic groups: cytochrome c1, the 'Rieske' iron-sulphur centre and the two b cytochromes. It is apparent that firstly, the circular dichroism (CD) properties of cytochrome c1 within the bc1 complex differ from those found in the isolated cytochrome c1 and secondly, both the oxidized and reduced b cytochromes exhibit an intense spectrum of bilobic shape, with the wavelengths of the cross-over points closely corresponding to those of the maxima in the optical absorbance spectra. These latter CD features are discussed in relation to the proposed structure of cytochrome b.
从牛心分离得到的线粒体细胞色素bc1复合物的圆二色光谱已被解析为各个辅基的贡献：细胞色素c1、“里斯克”铁硫中心和两个b型细胞色素。显然，首先，bc1复合物中的细胞色素c1的圆二色性（CD）特性与分离出的细胞色素c1中的不同；其次，氧化态和还原态的b型细胞色素均呈现出强烈的双叶形光谱，交叉点的波长与光吸收光谱中的最大值波长密切对应。后一种CD特征将结合细胞色素b的推测结构进行讨论。
