Aagaard Josephine B, Bjerrum Karen B, Graversen Jonas H, Henriksen Maiken L, Hansen Soren W K
Institute of Molecular Medicine, University of Southern Denmark , Odense, Denmark .
Monoclon Antib Immunodiagn Immunother. 2018 Nov;37(5):207-211. doi: 10.1089/mab.2018.0024.
Collectin LK (CL-LK) is a recently described collectin complex, which upon binding to microbial glycoconjugates, activates the lectin pathway of the complement system, and thereby contributes to the removal of invading microorganisms. The complex consists of the two collectins; Collectin K1 (kidney 1 alias CL-11) and Collectin L1 (liver 1 alias CL-10). At present, most efforts have been made on the characterization of CL-K1, and little is known about the function of CL-L1 and its association with diseases. Deficiency of either of the two collectins is associated with the developmental syndrome 3MC, whereas increased plasma levels of CL-K1 are associated with disseminated intravascular coagulation. Using CL-LK purified from human plasma as an immunogen, we succeed in generating seven monoclonal antibodies (mAbs) with specificity for CL-L1. All seven mAbs recognize both native and recombinant CL-L1. In addition, four of the mAbs were successful in immunohistochemical detection of CL-L1 in human tissues. To our knowledge, these are the first mAbs specific for human native CL-L1 described in the literature, and we expect them to be of great importance in characterizing the function of CL-L1, as well as for the study of CL-L1's association with disease.
凝集素LK（CL-LK）是一种最近被描述的凝集素复合物，它在与微生物糖缀合物结合后，激活补体系统的凝集素途径，从而有助于清除入侵的微生物。该复合物由两种凝集素组成：凝集素K1（肾脏1，别名CL-11）和凝集素L1（肝脏1，别名CL-10）。目前，大多数研究都集中在CL-K1的特性研究上，而关于CL-L1的功能及其与疾病的关联知之甚少。这两种凝集素中的任何一种缺乏都与3MC发育综合征有关，而CL-K1血浆水平升高与弥散性血管内凝血有关。我们以从人血浆中纯化的CL-LK作为免疫原，成功制备了7种对CL-L1具有特异性的单克隆抗体（mAb）。所有7种mAb都能识别天然和重组的CL-L1。此外，其中4种mAb成功地在人体组织中通过免疫组织化学检测到了CL-L1。据我们所知，这些是文献中首次描述的针对人天然CL-L1的特异性mAb，我们预计它们在表征CL-L1的功能以及研究CL-L1与疾病的关联方面将具有重要意义。
