Son Ah Reum, Jeong Jin-Young, Park Kyu Ree, Kim Minseok, Lee Sung Dae, Yoo Ji-Hyock, Do Yoon-Jung, Reddy Kondreddy Eswar, Lee Hyun-Jeong
Department of Animal Science and Technology, Konkuk University, Seoul, Republic of Korea.
Animal Nutritional Physiology Team, National Institute of Animal Science, Rural Development Administration, Wanju-gun, Republic of Korea.
PeerJ. 2018 Oct 19;6:e5791. doi: 10.7717/peerj.5791. eCollection 2018.
The experiment was conducted to determine the effects of graded dietary selenium (Se) on organ weight and Se concentrations in tissues and to develop equations for estimating dietary Se intake in pigs. Sixteen barrows (initial body weight = 30.0 ± 2.6) were allotted to four dietary treatments including graded Se supplementations with 0, 1, 5, and 50 mg/kg of diet. The experimental diets fed to the pigs for 30 d, and then the pigs were euthanized, and the organs, muscle, and urine samples were collected. The hair and blood samples of pigs were collected on d 15 and 30. Equations were developed for predicting daily Se intake using the Se concentration in plasma, hair, liver, kidneys, muscle, or urine. For graded dietary Se concentrations, linear and quadratic effects on the final body weight, weight and relative weight of liver and kidneys were not observed. The Se concentration in plasma, hair, liver, kidneys, muscle, and urine were linearly and quadratically increased as dietary Se concentration increased ( < 0.001). The dietary Se concentration was positively correlated with the Se concentrations in the plasma, organs, muscle, and urine ( > 0.81, < 0.001). The equations for estimating dietary Se intake using the Se concentration in the plasma, hair, or organ as an independent variable were significant ( < 0.05). In conclusion, the dietary Se concentration was well reflected in the Se concentration in the plasma, hair, liver, kidneys, and urine. The Se concentration in the plasma, hair, liver, and kidneys can be used as an independent variable for estimating the Se intake.
进行该实验是为了确定分级日粮硒（Se）对猪器官重量和组织中硒浓度的影响，并建立估算猪日粮硒摄入量的方程。16头公猪（初始体重 = 30.0 ± 2.6）被分配到四种日粮处理组，包括分别添加0、1、5和50 mg/kg日粮的分级硒补充剂。给猪饲喂实验日粮30天，然后对猪实施安乐死，并采集器官、肌肉和尿液样本。在第15天和第30天采集猪的毛发和血液样本。利用血浆、毛发、肝脏、肾脏、肌肉或尿液中的硒浓度建立了预测每日硒摄入量的方程。对于分级日粮硒浓度，未观察到对最终体重、肝脏和肾脏重量及相对重量的线性和二次效应。随着日粮硒浓度的增加，血浆、毛发、肝脏、肾脏、肌肉和尿液中的硒浓度呈线性和二次增加（P < 0.001）。日粮硒浓度与血浆、器官、肌肉和尿液中的硒浓度呈正相关（r > 0.81，P < 0.001）。以血浆、毛发或器官中的硒浓度作为自变量估算日粮硒摄入量的方程具有显著性（P < 0.05）。总之，日粮硒浓度在血浆、毛发、肝脏、肾脏和尿液中的硒浓度中得到了很好的体现。血浆、毛发、肝脏和肾脏中的硒浓度可作为估算硒摄入量的自变量。
