抑制血清反应因子通过调节 Egr-1 和上皮-间充质转化抑制宫颈癌系的侵袭。
Suppressing serum response factor inhibits invasion in cervical cancer cell lines via regulating Egr‑1 and epithelial-mesenchymal transition.
机构信息
Clinical Skills Training Center, Henan University of Chinese Medicine, Zhengzhou, Henan 450046, P.R. China.
Perinatal Care Division, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, P.R. China.
出版信息
Int J Mol Med. 2019 Jan;43(1):614-620. doi: 10.3892/ijmm.2018.3954. Epub 2018 Oct 24.
Serum response factor (SRF) is a transcription factor that has important roles in tumor progression. However, its role in cervical cancer cell proliferation and invasion remains unclear. The present study revealed that SRF silencing constrained cervical cancer cell proliferation and invasion via controlling early growth response‑1 (Egr‑1). The results demonstrated that SRF was significantly increased in cervical cancer tissues and cell lines, compared with normal. Suppressing SRF, by using a loss‑of‑function experiment, constrained cervical cancer cell proliferation, invasion, and epithelial‑mesenchymal transition. Furthermore, SRF knockdown significantly downregulated Egr‑1 expression in cervical cancer cell lines, and overexpression of Egr‑1 reversed the effect of SRF on cell proliferation, invasion, and epithelial‑mesenchymal transition. Therefore, SRF may control cell proliferation and invasion by regulating Egr‑1 in cervical cancer.
血清反应因子（SRF）是一种转录因子，在肿瘤进展中具有重要作用。然而，其在宫颈癌细胞增殖和侵袭中的作用尚不清楚。本研究表明，通过控制早期生长反应因子-1（Egr-1），SRF 沉默可抑制宫颈癌细胞的增殖和侵袭。结果表明，与正常组织相比，SRF 在宫颈癌组织和细胞系中显著增加。通过功能丧失实验抑制 SRF 可抑制宫颈癌细胞的增殖、侵袭和上皮-间充质转化。此外，SRF 敲低显著下调了宫颈癌细胞系中 Egr-1 的表达，而过表达 Egr-1 则逆转了 SRF 对细胞增殖、侵袭和上皮-间充质转化的影响。因此，SRF 可能通过调节宫颈癌中的 Egr-1 来控制细胞增殖和侵袭。
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