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完整肝细胞中L型丙酮酸激酶的磷酸化。响应胰高血糖素和Ca2+偶联激动剂去氧肾上腺素时磷酸化位点的定位。

Phosphorylation of type-L pyruvate kinase in intact hepatocytes. Localisation of the phosphorylation site in response to both glucagon and the Ca2+-linked agonist phenylephrine.

作者信息

Hsu Y C, Bloxham D P, Giles I G

出版信息

FEBS Lett. 1987 Jun 22;218(1):1-6. doi: 10.1016/0014-5793(87)81006-2.

DOI:10.1016/0014-5793(87)81006-2
PMID:3036585
Abstract

Pyruvate kinase is one of the enzymes which can be phosphorylated by stimulation of the cell with either glucagon or Ca2+-linked hormones. Whether these two classes of hormones phosphorylate the same site on the enzyme is unclear. Our results demonstrate that isolation of [32P]phosphorylated type-L pyruvate kinase from glucagon-treated hepatocytes followed by aspartyl-prolyl cleavage yields a [32P]phosphorylated peptide of Mr 17,000. This fragment is also phosphorylated in response to the Ca2+-mediated agonist phenylephrine.

摘要

丙酮酸激酶是在细胞受到胰高血糖素或与Ca2+相关的激素刺激时会被磷酸化的酶之一。这两类激素是否使该酶的同一位点磷酸化尚不清楚。我们的结果表明，从用胰高血糖素处理的肝细胞中分离出[32P]磷酸化的L型丙酮酸激酶，然后进行天冬氨酰-脯氨酰切割，可产生一个分子量为17,000的[32P]磷酸化肽段。该片段在对Ca2+介导的激动剂去氧肾上腺素的反应中也会被磷酸化。

相似文献

1
Phosphorylation of type-L pyruvate kinase in intact hepatocytes. Localisation of the phosphorylation site in response to both glucagon and the Ca2+-linked agonist phenylephrine.完整肝细胞中L型丙酮酸激酶的磷酸化。响应胰高血糖素和Ca2+偶联激动剂去氧肾上腺素时磷酸化位点的定位。
FEBS Lett. 1987 Jun 22;218(1):1-6. doi: 10.1016/0014-5793(87)81006-2.
2
Evidence for the activation of the multifunctional Ca2+/calmodulin-dependent protein kinase in response to hormones that increase intracellular Ca2+.
J Biol Chem. 1987 Jul 25;262(21):10154-63.
3
Glucagon and the Ca2+-linked hormones angiotensin II, norepinephrine, and vasopressin stimulate the phosphorylation of distinct substrates in intact hepatocytes.
J Biol Chem. 1982 Nov 10;257(21):13135-43.
4
A study of the mechanism of glucagon-induced protein phosphorylation in isolated rat hepatocytes using (Sp)-cAMPS and (Rp)-cAMPS, the stimulatory and inhibitory diastereomers of adenosine cyclic 3',5'-phosphorothioate.一项使用腺苷环3',5'-硫代磷酸的刺激性和抑制性非对映体（Sp）-cAMPS和（Rp）-cAMPS对分离的大鼠肝细胞中胰高血糖素诱导的蛋白质磷酸化机制进行的研究。
J Biol Chem. 1987 Mar 25;262(9):4324-32.
5
Phosphorylation of L-type pyruvate kinase by a Ca2+/calmodulin-dependent protein kinase.L型丙酮酸激酶被一种钙调蛋白依赖性蛋白激酶磷酸化。
J Biol Chem. 1985 Oct 25;260(24):13018-22.
6
Modulation of the phosphorylation state of rat liver pyruvate kinase by allosteric effectors and insulin.变构效应剂和胰岛素对大鼠肝脏丙酮酸激酶磷酸化状态的调节
J Biol Chem. 1979 Aug 25;254(16):7855-64.
7
The role of calcium ion as a mediator of the effects of angiotensin II, catecholamines, and vasopressin on the phosphorylation and activity of enzymes in isolated hepatocytes.钙离子作为血管紧张素II、儿茶酚胺和血管加压素对分离肝细胞中酶的磷酸化和活性影响的介质的作用。
J Biol Chem. 1979 Aug 10;254(15):7147-56.
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The glucagon-induced activation of pyruvate dehydrogenase in hepatocytes is diminished by 4 beta-phorbol 12-myristate 13-acetate. A role for cytoplasmic Ca2+ in dehydrogenase regulation.4β-佛波醇12-肉豆蔻酸酯13-乙酸酯可减弱胰高血糖素诱导的肝细胞丙酮酸脱氢酶激活。细胞质Ca2+在脱氢酶调节中的作用。
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9
Glucocorticoid hormones have a permissive role in the phosphorylation of L-type pyruvate kinase by glucagon.糖皮质激素在胰高血糖素介导的L型丙酮酸激酶磷酸化过程中起允许作用。
Eur J Biochem. 1982 May;124(1):103-8. doi: 10.1111/j.1432-1033.1982.tb05911.x.
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The role of Ca2+ and cyclic AMP in the phosphorylation of rat-liver soluble proteins by endogenous protein kinases.钙离子和环磷酸腺苷在大鼠肝脏可溶性蛋白由内源性蛋白激酶磷酸化过程中的作用。
Eur J Biochem. 1980 Dec;113(1):131-40. doi: 10.1111/j.1432-1033.1980.tb06147.x.

引用本文的文献

1
Epidermal-growth-factor stimulation of gluconeogenesis in isolated rat hepatocytes involves the inactivation of pyruvate kinase.表皮生长因子对离体大鼠肝细胞糖异生的刺激作用涉及丙酮酸激酶的失活。
Biochem J. 1988 Oct 1;255(1):361-4.
2
Insulin regulation of pyruvate kinase activity in cultured rat hepatocytes, in the presence of vasopressin, ionophore A23187 or 4 beta-phorbol 12 beta-myristate 13 alpha-acetate.在存在血管加压素、离子载体A23187或4β-佛波醇12β-肉豆蔻酸酯13α-乙酸酯的情况下，胰岛素对培养的大鼠肝细胞中丙酮酸激酶活性的调节作用。
Biochem J. 1988 Jun 15;252(3):717-21. doi: 10.1042/bj2520717.