表皮生长因子对离体大鼠肝细胞糖异生的刺激作用涉及丙酮酸激酶的失活。
Epidermal-growth-factor stimulation of gluconeogenesis in isolated rat hepatocytes involves the inactivation of pyruvate kinase.
作者信息
Moule S K, McGivan J F
机构信息
Department of Biochemistry, School of Medical Sciences, University Walk, Bristol, U.K.
出版信息
Biochem J. 1988 Oct 1;255(1):361-4.
Abstract
Preincubation of rat hepatocytes with EGF (epidermal growth factor) caused a stimulation of gluconeogenesis from alanine. The effect was maximal after preincubation of 20 min, and a half-maximal effect of EGF was obtained at 10 nM. EGF also stimulated gluconeogenesis from lactate and asparagine, but not from glutamine or from proline. Preincubation of hepatocytes with EGF caused a stable inactivation of pyruvate kinase, which may account, at least in part, for the observed effects of EGF on gluconeogenesis.
摘要
用表皮生长因子（EGF）对大鼠肝细胞进行预孵育，会刺激丙氨酸的糖异生作用。预孵育20分钟后效果达到最大，10 nM的EGF可产生半数最大效应。EGF还能刺激乳酸和天冬酰胺的糖异生作用，但对谷氨酰胺或脯氨酸无此作用。用EGF对肝细胞进行预孵育会导致丙酮酸激酶稳定失活，这可能至少部分解释了EGF对糖异生作用的观察效果。
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本文引用的文献
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FEBS Lett. 1980 Oct 6;119(2):271-4. doi: 10.1016/0014-5793(80)80269-9.
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Epidermal growth factor stimulates monovalent cation transport in isolated rat hepatocytes.
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Distinct effects of glucagon and vasopressin on proline metabolism in isolated hepatocytes. The role of oxoglutarate dehydrogenase.胰高血糖素和血管加压素对分离肝细胞中脯氨酸代谢的不同作用。草酰戊二酸脱氢酶的作用。
Biochem J. 1984 Jan 15;217(2):477-83. doi: 10.1042/bj2170477.
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Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen.使用带有非致癌显色剂的氧化酶-过氧化物酶系统测定血糖。
J Clin Pathol. 1969 Mar;22(2):158-61. doi: 10.1136/jcp.22.2.158.
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Arch Biochem Biophys. 1974 Oct;164(2):518-26. doi: 10.1016/0003-9861(74)90062-9.
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Ca2+-dependent activation of oxoglutarate dehydrogenase by vasopressin in isolated hepatocytes.血管加压素对分离肝细胞中依赖钙离子的α-酮戊二酸脱氢酶的激活作用。
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Epidermal growth factor and angiotensin II stimulate formation of inositol 1,4,5- and inositol 1,3,4-trisphosphate in hepatocytes. Differential inhibition by pertussis toxin and phorbol 12-myristate 13-acetate.表皮生长因子和血管紧张素II刺激肝细胞中肌醇1,4,5-三磷酸和肌醇1,3,4-三磷酸的形成。百日咳毒素和佛波醇12-肉豆蔻酸酯13-乙酸酯的差异抑制作用。
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