Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Fish Shellfish Immunol. 2019 Jan;84:541-550. doi: 10.1016/j.fsi.2018.10.047. Epub 2018 Oct 23.
Shrimp in culture ponds are challenged by various pathogens as well as harsh water environment. The innate immune system and environmental stress response system of shrimp paly an important role in shrimp survival and growth. For remission the endoplasmic reticulum (ER)-stress caused by environmental stress, unfolded protein response (UPR) may reduce the synthesis of most proteins, including great mass of immune factors, which could weaken the immune function of shrimp. Therefore, how cells keep appropriate amount of immune factor synthesis under such a situation is critical important for shrimp health and growth. In this study, we cloned a new Crustin gene (LvCruU) from Litopenaeus vannamei. We showed that LvCruU has antibacterial activity, and reducing its expression would increase the cumulative mortality of L. vannamei upon the Vibrio parahemolyticus infection. In addition, we found that promoter activity of LvCruU was enhanced not only by the deformed epidermal autoregulatory factor-1 (Deaf1), but also by activating transcription factor 3 (LvATF3) of shrimp UPR. Real-time RT-PCR showed that LvCruU and LvATF3 both were induced upon UPR activation. And moreover, in Thapsigargin plus dsLvCruU injection test, we showed that down-regulation of LvCruU increased the cumulative mortality of V. parahemolyticus-infected shrimp under ER-stress. These results suggest that LvCruU work as a downstream effector of UPR, and contribute to antimicrobic immune response upon ER-stress in L. vannamei.
养殖池中的虾类面临着各种病原体和恶劣水环境的挑战。虾类的先天免疫系统和环境应激反应系统在虾类的生存和生长中起着重要作用。为了缓解环境应激引起的内质网（ER）应激，未折叠蛋白反应（UPR）可能会减少大多数蛋白质的合成，包括大量的免疫因子，这可能会削弱虾类的免疫功能。因此，细胞如何在这种情况下保持适当数量的免疫因子合成对于虾类的健康和生长至关重要。在这项研究中，我们从凡纳滨对虾中克隆了一个新的 Crustin 基因（LvCruU）。我们表明 LvCruU 具有抗菌活性，降低其表达会增加凡纳滨对虾在副溶血弧菌感染后的累积死亡率。此外，我们发现 LvCruU 的启动子活性不仅受到变形表皮自调节因子-1（Deaf1）的增强，还受到虾 UPR 的激活转录因子 3（LvATF3）的增强。实时 RT-PCR 显示，LvCruU 和 LvATF3 在 UPR 激活后均被诱导。而且，在 Thapsigargin 加 dsLvCruU 注射试验中，我们表明，在 ER 应激下，下调 LvCruU 会增加副溶血弧菌感染虾的累积死亡率。这些结果表明，LvCruU 作为 UPR 的下游效应因子，在凡纳滨对虾 ER 应激时有助于抗菌免疫反应。
