College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, 350002, China.
School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st Street, Gainesville, FL, 32653, USA.
Fish Shellfish Immunol. 2019 Jan;84:609-626. doi: 10.1016/j.fsi.2018.10.044. Epub 2018 Oct 23.
In recent years, the abalone aquaculture industry has been threatened by the deteriorating environmental conditions, such as hypoxia and thermal stress in the hot summers. It is necessary to investigate the molecular mechanism in response to these environmental challenges, and subsequently understand the immune defense system. In this study, the transcriptome profiles by RNA-seq of hemocytes from the small abalone Haliotis diversicolor after exposure to hypoxia, thermal stress, and hypoxia plus thermal stress were established. A total of 103,703,074 clean reads were obtained and 99,774 unigenes were assembled. Of the 99,774 unigenes, 47,154 and 20,455 had homologous sequences in the Nr and Swiss-Prot protein databases, while 16,944 and 10,840 unigenes could be classified by COG or KEGG databases, respectively. RNAseq analysis revealed that the differentially expressed genes (DEGs) after challenges of hypoxia, thermal stress, or hypoxia plus thermal stress were 24,189, 29,165 and 23,665, among which more than 3000 genes involved in at least 230 pathways, including several classical immune-related pathways. The genes and pathways that were involved in immune response to hypoxia/thermal challenges were identified by transcriptome analysis and further validated by quantitative real-time PCR and RNAi technology. The findings in this study can provide information on H. diversicolor innate immunity to improve the abalone aquaculture industry, and the analysis of the potential immune-related genes in innate immunity signaling pathways and the obtained transcriptome data can provide an invaluable genetic resource for the study of the genome and functional genes.
近年来，鲍鱼养殖业受到了环境恶化的威胁，如夏季缺氧和热应激。有必要研究应对这些环境挑战的分子机制，从而了解其免疫防御系统。在这项研究中，通过 RNA-seq 技术对小鲍鱼（Haliotis diversicolor）血细胞在缺氧、热应激以及缺氧加热应激暴露后的转录组图谱进行了分析。共获得 103,703,074 条清洁reads，并组装了 99,774 个 unigenes。在 99,774 个 unigenes中，有 47,154 个和 20,455 个分别在 Nr 和 Swiss-Prot 蛋白质数据库中有同源序列，而 16,944 个和 10,840 个 unigenes分别可以通过 COG 或 KEGG 数据库进行分类。RNAseq 分析显示，在缺氧、热应激或缺氧加热应激挑战后，差异表达基因（DEGs）分别为 24,189、29,165 和 23,665，其中有 3000 多个基因参与了至少 230 条通路，包括几个经典的免疫相关通路。通过转录组分析鉴定了参与免疫应对缺氧/热应激的基因和通路，并通过定量实时 PCR 和 RNAi 技术进一步验证。本研究为改善鲍鱼养殖业提供了小鲍鱼先天免疫的信息，对先天免疫信号通路中潜在的免疫相关基因的分析以及获得的转录组数据，为研究基因组和功能基因提供了宝贵的遗传资源。
