Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing 100193, PR China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Department of Applied Chemistry, China Agricultural University, Beijing 100193, PR China.
Ecotoxicol Environ Saf. 2019 Jan 15;167:404-411. doi: 10.1016/j.ecoenv.2018.10.020. Epub 2018 Oct 23.
An enantioselective method of pyriproxyfen enantiomers in fertilized soil was established on Lux Cellulose-3 column using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The effects of urea, potassium dihydrogen phosphate, organic fertilizer and compound fertilizer on the dissipation of pyriproxyfen in soil were investigated. Studies have shown that pyriproxyfen had a good linearity in the range of 0.050-7.5 μg/g. The method LODs and LOQs of two enantiomers were 0.0032-0.015 μg/g and 0.050 μg/g, respectively. The recoveries of two enantiomers ranged from 80.61% to 110.86%, intra-day together with inter-day RSDs of 1.38-12.52%. The results indicated that compound fertilizer could promote the dissipation of pyriproxyfen in soil, while the organic fertilizer, potassium dihydrogen phosphate and urea slowed down the dissipation of pyriproxyfen. Enantioselective dissipation of pyriproxyfen enantiomers occurred in blank soil and fertilized soil but performed not obviously. Pyriproxyfen almost not dissipated for 42 d when urea was added. These results revealed that the use of fertilizers would have impacts on the behaviors of this pesticide. The impacts were relatively complex which should be fully considered in future environmental risk evaluation.
采用手性 Lux Cellulose-3 柱，建立了超高效液相色谱-串联质谱法（UPLC-MS/MS）对吡丙醚对映异构体在施肥土壤中的手性分析方法。考察了尿素、磷酸二氢钾、有机肥和复混肥对土壤中吡丙醚消解的影响。研究表明，吡丙醚在 0.050-7.5μg/g 范围内具有良好的线性关系。两种对映异构体的方法检出限和定量限分别为 0.0032-0.015μg/g 和 0.050μg/g。两种对映异构体的回收率在 80.61%-110.86%之间，日内和日间 RSD 为 1.38%-12.52%。结果表明，复混肥能促进土壤中吡丙醚的消解，而有机肥、磷酸二氢钾和尿素则减缓了吡丙醚的消解。在空白土壤和施肥土壤中，吡丙醚对映异构体均发生了手性消解，但表现不明显。添加尿素时，吡丙醚在 42d 内几乎没有消解。这些结果表明，肥料的使用会影响到该农药的行为。这些影响较为复杂，在未来的环境风险评估中应充分考虑。
