School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Toxins (Basel). 2018 Oct 25;10(11):425. doi: 10.3390/toxins10110425.
In the current study, deterrent assay, contact bioassay, lethal concentration (LC) analysis and gene expression analysis were performed to reveal the repellent or insecticidal potential of oil against . oil demonstrated an excellent deterrence index (0.8) at 2 g/L after 48 h. The oil demonstrated a pronounced contact mortality rate (72%) at a dose of 4 g/L after 24 h. Probit analysis was performed to estimate LC-values of oil (40%) against (LC = 0.115 g/L and LC = 0.37 g/L respectively) after 24 h. Furthermore, to probe changes in gene expression due to oil contact in , the expression of , genes were examined at doses of LC and LC. Four out of the five selected genes-, and -showed upregulation at LC, whereas, gene showed maximum upregulation expression at LC. Finally, the major components of oil (terpinen-4-ol) were docked and MD simulated into the related proteins of the selected genes to explore ligand⁻protein modes of interactions and changes in gene expression. The results show that oil has remarkable insecticidal and deterrent effects and also has the ability to affect the reproduction and development in by binding to proteins.
在本研究中，进行了驱避剂测定、接触生物测定、致死浓度 (LC) 分析和基因表达分析，以揭示 油对 的驱避或杀虫潜力。 油在 48 小时后 2 g/L 时表现出极好的驱避指数（0.8）。在 24 小时时，4 g/L 的剂量下，油表现出明显的接触死亡率（72%）。进行了概率分析以估计 油（40%）对 的 LC 值（分别为 LC = 0.115 g/L 和 LC = 0.37 g/L）。此外，为了研究 油接触导致的基因表达变化，在 LC 和 LC 剂量下检查了 、 、 基因的表达。在五个选定基因中，有四个基因（-、-和-）在 LC 时表现出上调表达，而 基因在 LC 时表现出最大上调表达。最后，将 油的主要成分（松油烯-4-醇）对接并 MD 模拟到选定基因的相关蛋白中，以探索配体-蛋白相互作用方式和基因表达的变化。结果表明， 油具有显著的杀虫和驱避作用，并且还具有通过与蛋白质结合影响 繁殖和发育的能力。
