Reproductive Medical Center, the International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China..
Reproductive Medical Center, the International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
Reprod Biomed Online. 2018 Nov;37(5):590-599. doi: 10.1016/j.rbmo.2018.08.021. Epub 2018 Oct 6.
Does the adapted carrier Cryoplus improve the quality of cryopreserved spermatozoa compared with the use of conventional containers, and what is the effect of the adapted carrier on clinical outcomes?
Semen samples from 27 cases of oligozoospermia were used to investigate whether the adapted carrier improved cryopreserved sperm quality compared with the use of 0.25-ml straws and 2-ml cryogenic vials. Thirty testicular sperm samples were used to study the quality of testicular spermatozoa cryopreserved in the adapted carrier. The retrospective study included a further 104 men with azoospermia to investigate the clinical outcomes of testicular spermatozoa cryopreserved with the adapted carriers. Men with mostly obstructive azoospermia were included in this study.
The adapted carrier improved cryopreserved spermatozoa motility of semen samples compared with 2-ml cryogenic vials but not compared with 0.25-ml straws. No differences were found in cryopreserved sperm DNA fragmentation among the three carriers. Fertilization and good-quality embryo rates were similar in ICSI cycles using fresh or cryopreserved testicular spermatozoa. Additionally, no difference was evident between frozen-thawed embryo transfer cycles using fresh or cryopreserved testicular spermatozoa in clinical pregnancy, implantation, miscarriage, live birth rates or birth weight.
The adapted carrier improved the cryopreserved sperm motility compared with the effects of 2-ml cryogenic vials. The outcomes of intracytoplasmic sperm injection and frozen-thawed embryo transfer outcomes indicate that testicular spermatozoa cryopreserved using the adapted carrier is not inferior to fresh testicular spermatozoa. The use of the adapted carrier for cryopreserving human testicular spermatozoa especially from obstructive azoospermia is simple and effective.
与使用传统容器相比，改良载体 Cryoplus 是否能提高冷冻精子的质量，改良载体对临床结局有何影响？
使用 27 例少精子症患者的精液样本，研究改良载体是否能改善冷冻精子质量，与使用 0.25-ml straws 和 2-ml 低温冷冻管相比。使用 30 例睾丸精子样本研究改良载体中冷冻保存的睾丸精子质量。回顾性研究包括 104 例无精子症男性，研究改良载体冷冻保存睾丸精子的临床结局。本研究纳入的男性大多为梗阻性无精子症。
与 2-ml 低温冷冻管相比，改良载体提高了精液样本冷冻精子的活动力，但与 0.25-ml straws 相比无差异。三种载体的冷冻精子 DNA 碎片率无差异。使用新鲜或冷冻睾丸精子进行卵胞浆内单精子注射的 ICSI 周期的受精率和优质胚胎率相似。此外，新鲜和冷冻睾丸精子的冻融胚胎移植周期在临床妊娠、着床、流产、活产率或出生体重方面均无差异。
与 2-ml 低温冷冻管相比，改良载体提高了冷冻精子的活动力。ICSI 和冻融胚胎移植的结局表明，使用改良载体冷冻保存的睾丸精子并不逊于新鲜睾丸精子。改良载体用于冷冻保存人类睾丸精子，尤其是梗阻性无精子症患者的睾丸精子，操作简单、有效。
