Oya Y, Tonomura A, Yamamoto K
Int J Cancer. 1987 Jul 15;40(1):69-73. doi: 10.1002/ijc.2910400113.
Hydrogen peroxide (H2O2) was found to be a potent inducer of the production of the early antigen complex (EA) and/or virus capsid antigen (VCA), determined by Epstein-Barr virus (EBV). Maximum synthesis of EBV antigens was induced by 0.2 mM H2O2, 5 days after a single 10-min treatment, in both B95-8 cells (30.2%) and P3HR-I cells (17.4%). These induction frequencies by H2O2 of proteins encoded by the EBV genome were almost the same as those obtained by 10 min treatment with the potent inducers, 12-O-tetradecanoylphorbol-13-acetate (TPA) and teleocidin. In combination with n-butyrate (4mM), H2O2 showed an additive induction of EBV antigens (1.9- and 1.7-fold in B95-8 and P3HR-I cells, respectively) and was as efficient as TPA and teleocidin. H2O2 induced EBV antigens at a very low level (less than 1%) in Raji cells by itself, but it induced EBV antigens synergistically in combination with n-butyrate (about 25-fold). In combined treatments using H2O2, TPA and teleocidin, the combination of H2O2 and TPA or H2O2 and teleocidin showed additive effects on the induction of synthesis of EBV antigens, but the combination of TPA and teleocidin showed almost the same induction level as that produced by H2O2, TPA or teleocidin alone. The inducing activities of H2O2, TPA and teleocidin were suppressed completely, in the case of H2O2, and slightly, in the cases of TPA and teleocidin, by treatment with catalase. Moreover, the effects of H2O2 were largely suppressed by scavengers of hydroxyl radical (X OH) and singlet oxygen (1O2), but not by superoxide dismutase (SOD), whereas the induction of EBV proteins by TPA and teleocidin was largely suppressed by SOD, but only slightly by scavengers of X OH and 1O2. Thus, the biological actions of H2O2 on the activation of the EBV genome seem to be essentially different from those of TPA and teleocidin, though the biological actions of TPA and teleocidin may be partially ascribed to those of H2O2.
过氧化氢（H₂O₂）被发现是一种强力诱导剂，可诱导由爱泼斯坦 - 巴尔病毒（EBV）决定的早期抗原复合物（EA）和/或病毒衣壳抗原（VCA）的产生。在单次10分钟处理5天后，0.2 mM H₂O₂在B95 - 8细胞（30.2%）和P3HR - I细胞（17.4%）中均诱导出EBV抗原的最大合成量。H₂O₂对EBV基因组编码蛋白的这些诱导频率与用强力诱导剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）和远侧霉素处理10分钟所获得的频率几乎相同。与正丁酸盐（4 mM）联合使用时，H₂O₂对EBV抗原表现出累加诱导作用（在B95 - 8和P3HR - I细胞中分别为1.9倍和1.7倍），且与TPA和远侧霉素一样有效。H₂O₂单独在拉吉细胞中诱导EBV抗原的水平非常低（低于1%），但与正丁酸盐联合使用时可协同诱导EBV抗原（约25倍）。在使用H₂O₂、TPA和远侧霉素的联合处理中，H₂O₂与TPA或H₂O₂与远侧霉素的组合对EBV抗原合成的诱导表现出累加效应，但TPA和远侧霉素的组合所产生的诱导水平与单独使用H₂O₂、TPA或远侧霉素时几乎相同。过氧化氢酶处理可完全抑制H₂O₂的诱导活性，对于TPA和远侧霉素则略有抑制。此外，H₂O₂的作用在很大程度上被羟基自由基（·OH）和单线态氧（¹O₂）的清除剂抑制，但不被超氧化物歧化酶（SOD）抑制，而TPA和远侧霉素诱导EBV蛋白的作用在很大程度上被SOD抑制，但仅被·OH和¹O₂的清除剂轻微抑制。因此，H₂O₂对EBV基因组激活的生物学作用似乎与TPA和远侧霉素的本质不同，尽管TPA和远侧霉素的生物学作用可能部分归因于H₂O₂的作用。
