Murgia Maria V, Mogler Mark, Certoma Andrea, Green Diane, Monaghan Paul, Williams David T, Rowland Raymond R R, Gaudreault Natasha N
Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, 1800 Denison Avenue, Manhattan, KS, 66506, USA.
Merck Animal Health, 1102 Southern Hills Drive Ste.101, Ames, IA, 50010, USA.
Arch Virol. 2019 Feb;164(2):359-370. doi: 10.1007/s00705-018-4071-8. Epub 2018 Oct 26.
In this study, an alphavirus vector platform was used to deliver replicon particles (RPs) expressing African swine fever virus (ASFV) antigens to swine. Alphavirus RPs expressing ASFV p30 (RP-30), p54 (RP-54) or pHA-72 (RP-sHA-p72) antigens were constructed and tested for expression in Vero cells and for immunogenicity in pigs. RP-30 showed the highest expression in Vero cells and was the most immunogenic in pigs, followed by RP-54 and RP-sHA-p72. Pigs primed with two doses of the RP-30 construct were then boosted with a naturally attenuated ASFV isolate, OURT88/3. Mapping of p30 identified an immunodominant region within the amino acid residues 111-130. However, the principal effect of the prime-boost was enhanced recognition of an epitope covered by the peptide sequence 61-110. The results suggest that a strategy incorporating priming with a vector-expressed antigen followed by boosting with an attenuated live virus may broaden the recognition of ASFV epitopes.
在本研究中，使用了一种甲病毒载体平台将表达非洲猪瘟病毒（ASFV）抗原的复制子颗粒（RP）递送至猪体内。构建了表达ASFV p30（RP - 30）、p54（RP - 54）或pHA - 72（RP - sHA - p72）抗原的甲病毒RP，并在Vero细胞中测试其表达情况以及在猪体内的免疫原性。RP - 30在Vero细胞中表现出最高的表达水平，并且在猪体内具有最强的免疫原性，其次是RP - 54和RP - sHA - p72。用两剂RP - 30构建体进行初免的猪随后用天然减毒的ASFV分离株OURT88/3进行加强免疫。对p30的图谱分析确定了氨基酸残基111 - 130内的一个免疫显性区域。然而，初免 - 加强免疫的主要作用是增强了对由肽序列61 - 110覆盖的一个表位的识别。结果表明，一种先使用载体表达的抗原进行初免然后用减毒活病毒进行加强免疫的策略可能会拓宽对ASFV表位的识别。
