原代人骨骼肌细胞的电脉冲刺激
Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells.
作者信息
Nikolić Nataša, Aas Vigdis
机构信息
Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
Department of Life Sciences and Health, Faculty of Health Sciences, Oslo Metropolitan University, Oslo, Norway.
出版信息
Methods Mol Biol. 2019;1889:17-24. doi: 10.1007/978-1-4939-8897-6_2.
Electrical pulse stimulation (EPS) is an in vitro method of inducing contractions in cultured skeletal muscle cells of human and animal origin. Motor neuron activation of muscle fibers can be replaced by applying EPS on differentiated skeletal muscle cells (myotubes) in culture (Thelen et al. Biochemical J 321:845-848, 1997, Fujita et al. Exp Cell Res 313:1853-1865, 2007).Here we describe two protocols for EPS of human myotubes in 6-well plates: acute, high-frequency (single bipolar pulses of 2 ms, 100 Hz for 200 ms every fifth second for 5-60 min, 10-30 V) and chronic, low-frequency (single bipolar pulses of 2 ms, 1 Hz 10-30 V for 48 h) at the end of a 7 days long differentiation.
电脉冲刺激（EPS）是一种在体外诱导人和动物来源的培养骨骼肌细胞收缩的方法。通过对培养的分化骨骼肌细胞（肌管）施加电脉冲刺激，可以替代运动神经元对肌纤维的激活（Thelen等人，《生物化学杂志》321:845 - 848，1997；Fujita等人，《实验细胞研究》313:1853 - 1865，2007）。在此，我们描述两种在6孔板中对人肌管进行电脉冲刺激的方案：急性高频刺激（2毫秒的单极双相脉冲，每五秒一次，100赫兹，持续200毫秒，共5 - 60分钟，电压10 - 30伏）和慢性低频刺激（2毫秒的单极双相脉冲，1赫兹，电压10 - 30伏，持续48小时），刺激在7天的分化期结束时进行。
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