成肌过程中的转录组分析

Transcriptomic Profiling During Myogenesis.

作者信息

Majewska Alicja, Domoradzki Tomasz, Grzelkowska-Kowalczyk Katarzyna

机构信息

Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Warsaw, Poland.

出版信息

Methods Mol Biol. 2019;1889:127-168. doi: 10.1007/978-1-4939-8897-6_9.

DOI:10.1007/978-1-4939-8897-6_9

PMID:30367413

Abstract

Microarray-based transcriptomic profiling enables simultaneous measurement of expression of multiple genes from one biological sample. Here we describe a detailed protocol, which serves to examine global gene expression using whole genome oligonucleotide microarrays. We also provide examples of bioinformatics tools, which are helpful in analyses and interpretation of microarray data, and propose further biological assays, to warrant conclusions drawn from transcriptomic signature.

摘要

基于微阵列的转录组分析能够同时测量来自一个生物样本的多个基因的表达。在此，我们描述了一个详细的方案，该方案用于使用全基因组寡核苷酸微阵列检测全局基因表达。我们还提供了生物信息学工具的示例，这些工具有助于微阵列数据的分析和解释，并提出了进一步的生物学检测方法，以确保从转录组特征得出的结论的可靠性。