Falayi Olufunke Olubunmi, Oyagbemi Ademola Adetokunbo, Omobowale Temidayo Olutayo, Ayodele Emmanuel Abiodun, Adedapo Aduragbenro Deborah, Yakubu Momoh Audu, Adedapo Adeolu Alex
Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
J Complement Integr Med. 2018 Oct 26;16(3):/j/jcim.2019.16.issue-3/jcim-2017-0176/jcim-2017-0176.xml. doi: 10.1515/jcim-2017-0176.
Background The use of plants for the treatment and prevention of diseases in man and his animals has led to a renewed scientific interest in the use of medicinal plants for therapeutic purposes. The nephroprotective properties of methanol stem bark extract of Abrus precatorius against gentamicin-induced renal damage in rats was evaluated in this study. Methods Thirty male Wistar rats were divided into five equal groups. Group A was the negative control group while B was the positive control group which received gentamicin 100 mg/kg intra-peritoneally for 6 days. Group C were pretreated with 100 mg/kg extract for the 3 days and then concurrently with gentamicin 100 mg/kg for 3 days and group D were pretreated with 200 mg/kg extract for 3 days and then concurrently with gentamicin 100 mg/kg for 3 days. Group E received gentamicin intraperitoneally for 6 days followed by administration of 200 mg/kg of the extract for 3 days. Blood samples, kidneys and kidney homogenates were collected for haematological, biochemical, histopathological and immunohistochemical analysis. Results The results showed that no significant haematological changes were noted. The groups treated with extract exhibited significant increase in body weight gain. While group B significantly exhibited focal areas of inflammation, fatty degeneration, congestion of vessels, tubular necrosis and glomerular atrophy, the lesions were mild with the treated groups. Treated groups exhibited a dose dependent significant decrease in serum creatinine, urea, XO, NO and Myeloperoxidase, AOPP, Protein carbonyl, H2O2 generated and MDA levels when compared with group B. There were significant dose dependent improvements in SOD, GST, GSH, Protein thiol, and non-protein thiol levels in the treated groups when compared with group B. In immunohistochemistry, Group B exhibited over expression of CRP and NF-κB levels, and marked reduction in expression of Bcl-2 while the reverse was seen in the groups treated with methanol extracts of Abrus precatorius. Conclusion The methanol extract of Abrus precatorius plays a vital role against gentamicin induced renal damage by reducing levels of renal markers of oxidative stress, inflammation and apoptosis, enhancing enzymatic and non enzymatic renal antioxidant system, alongside an increase in Bcl-2 and a decrease in NF-κB and CRP expressions.
背景 利用植物治疗和预防人类及其动物的疾病，引发了科学界对药用植物用于治疗目的的新兴趣。本研究评估了鸡骨草甲醇茎皮提取物对庆大霉素诱导的大鼠肾损伤的肾保护特性。方法 将30只雄性Wistar大鼠分为五组，每组数量相等。A组为阴性对照组，B组为阳性对照组，腹腔注射100mg/kg庆大霉素，连续6天。C组先用100mg/kg提取物预处理3天，然后与100mg/kg庆大霉素同时给药3天；D组先用200mg/kg提取物预处理3天，然后与100mg/kg庆大霉素同时给药3天。E组腹腔注射庆大霉素6天，随后给予200mg/kg提取物，连续3天。采集血样、肾脏及肾脏匀浆进行血液学、生化、组织病理学和免疫组化分析。结果 结果显示，未观察到明显的血液学变化。用提取物处理的组体重增加显著增加。B组显著出现局灶性炎症、脂肪变性、血管充血、肾小管坏死和肾小球萎缩，而处理组的病变较轻。与B组相比，处理组血清肌酐、尿素、XO、NO和髓过氧化物酶、AOPP、蛋白质羰基、H2O2生成量和MDA水平呈剂量依赖性显著降低。与B组相比，处理组SOD、GST、GSH、蛋白质巯基和非蛋白质巯基水平有显著的剂量依赖性改善。在免疫组化中，B组CRP和NF-κB水平过度表达，Bcl-2表达明显降低，而鸡骨草甲醇提取物处理组则相反。结论 鸡骨草甲醇提取物通过降低氧化应激、炎症和凋亡的肾脏标志物水平，增强酶促和非酶促肾脏抗氧化系统，同时增加Bcl-2表达，降低NF-κB和CRP表达，对庆大霉素诱导的肾损伤起着至关重要的作用。
