Maleysson Vincent, Page Guylène, Janet Thierry, Klein Ronald L, Haida Obélia, Maurin Anne, Richard Serge, Champeroux Pascal, Fauconneau Bernard
EA 3808, NEUVACOD, University of Poitiers France; Centre de Recherches Biologiques, CERB, Chemin de Montifault, 18800, Baugy, France.
EA 3808, NEUVACOD, University of Poitiers France.
Behav Brain Res. 2019 Feb 1;359:127-134. doi: 10.1016/j.bbr.2018.10.026. Epub 2018 Oct 24.
In order to contribute to a better knowledge on the relationship between amyloid and tau pathology, and electroencephalography (EEG) disturbances, the aim of this study was to evaluate the effects of injection of beta amyloid Abeta(1-42) peptide, tau (a recombinant AAV (Adeno-Associated Virus) containing the human transgene tau with the P301 L mutation on rats and the combination of both, on the power of brain's rhythm (delta, theta, alpha, beta and gamma waves) during the different sleep/wake states of animals by EEG recording. Currently, no preclinical studies explore the effect of the tau pathology on EEG. The experimentations were performed 3 weeks and 3 months post injections. Beta amyloid deposits and hyperphosphorylated Tau are observed by immunohistofluorescence, only in the hippocampus. Furthermore, using a radial arm water maze, the main effect was observed on working memory which was significantly impaired in Abeta-Tau group only 3 months post injections. However, on EEG, as early as the 3 week, an overall decrease of the EEG bands power was observed in the treated groups, particularly the theta waves during the rapid eye movement (REM) sleep. Beta amyloid was mainly involved in these perturbations. Obviously, EEG seems to be an interesting tool in the early diagnostic of amyloid and tau pathologies, with a good sensitivity and the possibility to perform a follow up during a large period.
为了更好地了解淀粉样蛋白与tau蛋白病理之间的关系以及脑电图（EEG）紊乱情况，本研究的目的是通过EEG记录来评估注射β淀粉样蛋白Aβ(1-42)肽、tau蛋白（一种含有人类转基因tau且带有P301 L突变的重组腺相关病毒（AAV））以及两者组合对动物在不同睡眠/觉醒状态下脑节律（δ波、θ波、α波、β波和γ波）功率的影响。目前，尚无临床前研究探讨tau蛋白病理对EEG的影响。实验在注射后3周和3个月进行。通过免疫荧光观察发现，仅在海马体中观察到β淀粉样蛋白沉积和过度磷酸化的Tau蛋白。此外，使用放射状臂水迷宫实验，仅在注射后3个月时观察到对工作记忆的主要影响，在Aβ-Tau组中工作记忆明显受损。然而，在EEG方面，早在3周时，在治疗组中就观察到EEG频段功率总体下降，尤其是在快速眼动（REM）睡眠期间的θ波。β淀粉样蛋白主要参与了这些干扰。显然，EEG似乎是早期诊断淀粉样蛋白和tau蛋白病理的一个有趣工具，具有良好的敏感性以及在较长时期内进行随访的可能性。
