College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
China Meat Research Center, Beijing Academy of Food Sciences, Beijing 100068, China.
Meat Sci. 2019 Feb;148:96-104. doi: 10.1016/j.meatsci.2018.10.008. Epub 2018 Oct 11.
Glycated nitrosohaemoglobin (G-NO-Hb) prepared from porcine blood cells was used in natural fermented Harbin dry sausages to evaluate their possible as an alternative for sodium nitrite (SN). The results show that the G-NO-Hb had no significant effect on the pH, water activity, and growth of total bacteria and lactic acid bacteria counts (P > 0.05). G-NO-Hb inhibited Enterobacteriaceae growth (P < 0.05), but this inhibition was lower than SN. The redness of sausages with 2 g/kg G-NO-Hb were almost the same as SN sausages (P > 0.05). The thiobarbituric acid reactive substances of G-NO-Hb samples had no differences when compared to SN samples except at 12 days. Residual nitrite in G-NO-Hb sausages was lower than SN samples. The content of individual volatile compounds, such as hexanal (lipid oxidation marker), was nearly three times lower for SN than for G-NO-Hb samples. Overall, G-NO-Hb reduced nitrite residue and inhibited lipid oxidation and Enterobacteriaceae growth of sausages, demonstrating great potential as a substitute for sodium nitrite in meat products.
从猪血细胞中制备的糖化亚硝基血红蛋白(G-NO-Hb)被用于天然发酵的哈尔滨干香肠中,以评估其作为亚硝酸钠(SN)替代品的可能性。结果表明,G-NO-Hb 对 pH 值、水分活度以及总细菌和乳酸菌计数的生长没有显著影响(P>0.05)。G-NO-Hb 抑制肠杆菌科的生长(P<0.05),但这种抑制作用低于 SN。添加 2g/kg G-NO-Hb 的香肠的红色与 SN 香肠几乎相同(P>0.05)。除了在第 12 天外,G-NO-Hb 样品的硫代巴比妥酸反应物质与 SN 样品没有差异。G-NO-Hb 香肠中的亚硝酸盐残留量低于 SN 样品。个别挥发性化合物的含量,如己醛(脂质氧化标志物),SN 样品比 G-NO-Hb 样品低近三倍。总的来说,G-NO-Hb 减少了香肠中的亚硝酸盐残留量,抑制了脂质氧化和肠杆菌科的生长,表明其作为肉制品中亚硝酸钠替代品具有很大的潜力。