State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Harbin 150090, PR China; Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.
Chemosphere. 2019 Feb;216:186-194. doi: 10.1016/j.chemosphere.2018.10.132. Epub 2018 Oct 18.
Previous study has demonstrated that microaerobic process is effective in nitrogen removal from the wastewater with high ammonium and low carbon to nitrogen ratio. In the microaerobic system, synergistic action of anammox, ammonia oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and denitrifiers was the key issues to remove nitrogen from the wastewater rich in ammonium. Temperature has a significant effect on specific growth rate and activity of various nitrogen removal functional bacteria. In this study, the effect of temperature (35 °C-15 °C) on nitrogen removal were investigated in an up-flow microaerobic sludge reactor (UMSR) at the HRT of 8 h and reflux ratio of 45. Above 71.2% of total nitrogen (TN) and 80.7% of NH removal efficiencies were observed at the temperature no less than 17 °C. With the temperature further decreasing to 15 °C, denitrifiers still dominant the UMSR, but AOB, NOB and Candidatus Brocadia as the predominant anammox bacteria were inhibited revealed by high throughput sequencing, resulting in the decrease of TN and NH removal to 39.7% and 61.8%, respectively. Fortunately, when the temperature rebounded to 20 °C, a higher TN and NH removal of 81.2% and 97.3% were obtained again in the UMSR.
先前的研究表明，微氧工艺可有效去除高氨氮和低碳氮比废水中的氮。在微氧系统中，厌氧氨氧化菌（Anammox）、氨氧化菌（AOB）、亚硝酸盐氧化菌（NOB）和反硝化菌的协同作用是从富含氨氮的废水中去除氮的关键。温度对各种氮去除功能菌的比生长速率和活性有显著影响。本研究在 HRT 为 8 h 和回流比为 45 的上流式微氧污泥反应器（UMSR）中，考察了温度（35°C-15°C）对氮去除的影响。温度不低于 17°C 时，总氮（TN）去除率达到 71.2%以上，NH4+去除率达到 80.7%。当温度进一步降至 15°C 时，反硝化菌仍然是 UMSR 的主要优势菌，但高通量测序显示，AOB、NOB 和 Candidatus Brocadia 作为主要的厌氧氨氧化菌受到抑制，导致 TN 和 NH4+去除率分别下降至 39.7%和 61.8%。幸运的是，当温度回升至 20°C 时，UMSR 中又获得了更高的 TN 和 NH4+去除率，分别为 81.2%和 97.3%。
