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伊利湖微囊藻毒素降解菌的分离与鉴定

Isolation and Characterization of Microcystin-Degrading Bacteria from Lake Erie.

作者信息

Krishnan Anjali, Zhang Yu-Qin, Mou Xiaozhen

机构信息

Biological Science Department, Kent State University, Kent, OH, 44242, USA.

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Tiantan Xi Li, Beijing, 100050, China.

出版信息

Bull Environ Contam Toxicol. 2018 Nov;101(5):617-623. doi: 10.1007/s00128-018-2468-4. Epub 2018 Oct 27.

Abstract

Heterotrophic bacteria are suggested as the major agents that degrade microcystins (MCs), a major cyanotoxins, in natural environments. However, little is known of the taxonomic and functional diversity of MC-degrading bacteria in Lake Erie of the Laurentian Great Lakes, the largest freshwater system on earth. This study obtained six bacterial pure isolates from Lake Erie with an ability to use MCs as the sole carbon and energy sources. MC degradation rates of the isolates were impacted by temperature and pH. The key gene for MC degradation (mlrA) were failed to be PCR amplified from for all 6 MC degraders, indicating they may possess a novel MC degradation pathway. In addition for potentials used in MC bioremediation, two isolates maybe can offer extra benefits as biofertilizers.

摘要

异养细菌被认为是在自然环境中降解微囊藻毒素(MCs,一种主要的蓝藻毒素)的主要媒介。然而,对于地球上最大的淡水系统—— Laurentian五大湖的伊利湖中的MC降解细菌的分类和功能多样性,人们知之甚少。本研究从伊利湖获得了6株细菌纯分离株,它们能够将MCs作为唯一的碳源和能源。这些分离株的MC降解率受温度和pH值的影响。对所有6株MC降解菌进行PCR扩增,均未成功扩增出MC降解关键基因(mlrA),这表明它们可能拥有一种新的MC降解途径。此外,对于用于MC生物修复的潜力而言,有两株分离株可能作为生物肥料提供额外的益处。

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