Cui Guizhong, Suo Shengbao, Wang Ran, Qian Yun, Han Jing-Dong J, Peng Guangdun, Tam Patrick P L, Jing Naihe
State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Dev Growth Differ. 2018 Oct;60(8):463-472. doi: 10.1111/dgd.12568.
Gastrulation is a key milestone in early mouse development when multipotent epiblast cells are allocated to progenitors of diverse tissue lineages that constitute the ensemble of building blocks of the body plan. The analysis of gene function revealed that the activity of transcription factors is likely to be the fundamental driving force underpinning the lineage specification and tissue patterning in the primary germ layers. The developmental-spatial transcriptome of the gastrulating embryo revealed the concerted and interactive activity of the gene regulatory network anchored by development-related transcription factors. The findings of the network structure offer novel insights into the regionalization of tissue fates and enable tracking of the progression of epiblast patterning, leading to the construction of molecularly annotated fate maps of epiblast during gastrulation.
原肠胚形成是小鼠早期发育中的一个关键里程碑，此时多能上胚层细胞被分配到构成身体蓝图构建模块集合的不同组织谱系的祖细胞中。基因功能分析表明，转录因子的活性可能是支持原肠胚层中谱系特化和组织模式形成的基本驱动力。原肠胚形成期胚胎的发育空间转录组揭示了由发育相关转录因子锚定的基因调控网络的协同和交互活性。网络结构的研究结果为组织命运的区域化提供了新的见解，并能够追踪上胚层模式形成的进程，从而构建原肠胚形成期上胚层的分子注释命运图谱。
