Sirtuin3 deficiency exacerbates carbon tetrachloride-induced hepatic injury in mice.

Sirtuin3 (SIRT3) plays an important role in maintaining normal mitochondrial function and alleviating oxidative stress. After carbon tetrachloride (CCl ) administration, the expression of SIRT3 decreased in the liver of mice, which indicated that the SIRT3 might play a crucial role during chemical-induced acute hepatic injury. To verify the hypothesis, CCl was given to induce acute hepatic injury in SIRT3 knockout (KO) mice and wild-type (WT) mice. CCl -induced liver injury was more severe in SIRT3 KO mice compared with the WT mice. In addition, the oxidative stress induced by CCl was enhanced in the SIRT3 KO mice. Furthermore, the increased expression of dynamin-related protein 1 was also aggravated in SIRT3 KO mice after CCl administration. In conclusion, our study demonstrated that SIRT3 deficiency exacerbated CCl -induced impairment of the liver in mice, and the mechanism might be related to enhanced oxidative stress.