拮抗污斑稻病原菌活性及 BAS23 的促生活性
Antagonistic Activity against Dirty Panicle Rice Fungal Pathogens and Plant Growth-Promoting Activity of BAS23.
机构信息
Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Microbial Biotechnology Unit, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
出版信息
J Microbiol Biotechnol. 2018 Sep 28;28(9):1527-1535. doi: 10.4014/jmb.1804.04025.
Bacterial strain BAS23 was isolated from rice field soil and identified as . Based on dual culture method results, the bacterium BAS23 exhibited potent in vitro inhibitory activity on mycelial growth against a broad range of dirty panicle fungal pathogens of rice ( and ). Cell-free culture of BAS23 displayed a significant effect on germ tube elongation and mycelial growth. The highest dry weight reduction (%) values of and were 92.7%, 75.7% and 68.9%, respectively. Analysis of electrospray ionization-mass spectrometry (ESI-MS) and 1H nuclear magnetic resonance (NMR) spectroscopy revealed that the lipopeptides were iturin A with a C14 side chain (C14 iturinic acid) and with a C15 side chain (C15 iturinic acid), which were produced by BAS23 when it was cultured in nutrient broth (NB) for 72 h at 30°C.BAS23, the efficient antagonistic bacterium, also possessed in vitromultipletraits for plant growth promotionand improved rice seedling growth. The results indicated that B.amyloliquefaciens BAS23 represents a useful option either for biocontrol or as a plant growth-promoting agent.
从稻田土壤中分离得到细菌菌株 BAS23，并鉴定为 。根据双培养法的结果，该细菌 BAS23 对水稻多种污穂真菌病原菌（ 和 ）的菌丝生长具有很强的体外抑制活性。BAS23 的无细胞培养对芽管伸长和菌丝生长有显著的抑制作用。 和 的最高干重减少（%）值分别为 92.7%、75.7%和 68.9%。电喷雾电离-质谱（ESI-MS）和 1H 核磁共振（NMR）波谱分析表明，脂肽为iturin A 具有 C14 侧链（C14 iturinic acid）和 C15 侧链（C15 iturinic acid），这是 BAS23 在 30°C 下培养营养肉汤（NB）72 h 时产生的。BAS23 是一种有效的拮抗细菌，还具有促进植物生长和改善水稻幼苗生长的多种特性。结果表明，解淀粉芽孢杆菌 BAS23 是一种有前途的生物防治或植物生长促进剂。
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