University of Belgrade, Innovation Center of Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, Belgrade, Serbia.
University of Belgrade, Faculty of Technology and Metallurgy, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, Belgrade, Serbia.
Microb Pathog. 2018 Jul;120:71-78. doi: 10.1016/j.micpath.2018.04.056. Epub 2018 Apr 27.
In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusarium graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
在当前的研究中，我们认识到了一种新型海洋芽孢杆菌（Bacillus sp.）PPM3 的生物防治潜力。这种新型菌株是从埃及赫尔格达红海海洋沉积物中分离出来的 32 个分离物中筛选出来的，因为它能够抑制四种植物病原真菌的生长：黄曲霉（Aspergillus flavus）、禾谷镰刀菌（Fusarium graminearum）、毛霉（Mucor sp.）和链格孢菌（Alternaria sp.）。新型海洋菌株通过表型和分子方法进行了鉴定和特征描述。芽孢杆菌（Bacillus sp.）PPM3 的培养液滤液在体外抑制了所有测试真菌的生长和孢子萌发，对毛霉（Mucor sp.）的抑制率最高，达到 97.5%。该菌株培养液滤液的抑菌作用是由于产生了高度稳定的次级代谢产物，这些产物能够抵抗极端 pH 值、温度和酶处理。PCR 分析证实了参与合成抑菌脂肽的基因的表达：伊枯草菌素、杆菌霉素 D、杆菌肽和表面活性剂。在温室试验中，PPM3 菌株有效地降低了玉米植株中禾谷镰刀菌的发病率，并表现出额外的植物生长刺激作用。结果表明，新型海洋菌株 PPM3 可能具有商业应用的潜力，可作为防治土壤传播和采后病原真菌引起的各种植物病害的生物防治剂。
