同型半胱氨酸处理人视网膜细胞中环状 RNA 分子的表达分析。
Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine.
机构信息
a Eye and Vision Science Laboratory, Department of Physiology , University of Louisville School of Medicine , Louisville , Kentucky , USA.
b Department of Physiology , University of Louisville School of Medicine , Louisville , Kentucky , USA.
出版信息
Curr Eye Res. 2019 Mar;44(3):287-293. doi: 10.1080/02713683.2018.1542005. Epub 2018 Dec 5.
Abstract
PURPOSE
To characterize the global profile of circular RNAs (circRNAs) and their differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a line of human retinal pigment epithelial (RPE) cells.
MATERIALS AND METHODS
We treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on circRNA expression levels using dedicated human circRNA microarrays.
RESULTS
A total of 12,233 circRNAs were identified out of them 54 were differentially expressed (17 were down-regulated, and 37 were up-regulated) with a fold change >2.0 (p < 0.05) in Hcy-treated versus untreated cells.
CONCLUSIONS
To our knowledge, this is the first report profiling circRNAs in human RPE cells post-Hcy treatment mimicking hyperhomocysteinemic (HHcy) conditions that negatively affect retinal biology and vision. These findings are of potential clinical significance as they will help understand Hcy metabolism and HHcy-mediated diseases and identify potential diagnostic and therapeutic targets for eye diseases that are caused by elevated Hcy concentrations.
摘要
目的
分析同型半胱氨酸（Hcy）处理的人视网膜色素上皮（RPE）细胞系 ARPE-19 中环状 RNA（circRNA）的全球特征及其差异表达水平。
材料与方法
用 Hcy 处理 ARPE-19 细胞，采用特定的人环状 RNA 微阵列检测 Hcy 对 circRNA 表达水平的影响。
结果
共鉴定出 12233 个 circRNA，其中 54 个circRNA（17 个下调，37 个上调）在 Hcy 处理组与未处理组之间的差异倍数 >2.0（p < 0.05）。
结论
据我们所知，这是首次在模拟高同型半胱氨酸血症（HHcy）条件下，即 Hcy 处理后对人 RPE 细胞 circRNA 进行特征分析的研究。这些发现具有潜在的临床意义，因为它们将有助于理解 Hcy 代谢和 HHcy 介导的疾病，并为因 Hcy 浓度升高而引起的眼部疾病确定潜在的诊断和治疗靶点。
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