Okuda H, Obata H, Nakanishi T, Furukawa R, Hashimoto E
J Hepatol. 1987 Jun;4(3):357-63. doi: 10.1016/s0168-8278(87)80546-9.
We measured plasma abnormal prothrombin (des-gamma-carboxy prothrombin; DCP) levels in normal subjects and in patients with hepatocellular carcinoma and other various diseases using the enzyme-linked immunosorbent assay developed by Motohara et al. (Pediatr Res 1985; 19: 354-357). Fifty-eight percent of 52 patients with hepatocellular carcinoma had elevated DCP levels; 24 of 28 patients with advanced or moderately advanced hepatocellular carcinoma were positive. By contrast, 50 normal controls, 13 pregnant women and 10 patients with acute hepatitis had normal levels. Three of 55 patients with chronic liver disease, and 6 of 32 patients with other malignancies, showed a slight increase. Thus, increased plasma DCP appears useful for the diagnosis of hepatocellular carcinoma. To elucidate the mechanism for the increase of DCP in hepatocellular carcinoma, we cultured a human hepatoma cell line, huH-2, and measured the levels of this abnormal prothrombin in the medium. The huH-2 cells produced large amounts of DCP in the medium without added vitamin K. It increased in a cell concentration- and time-dependent fashion. These cells produced no detectable amount of DCP in the medium with added vitamin K. Thus, human hepatoma cell line huH-2 produces DCP, and its production is dependent on the amount of vitamin K available in the medium. Des-gamma-carboxy prothrombin may be a useful tumor marker for the diagnosis of hepatocellular carcinoma.
我们使用Motohara等人开发的酶联免疫吸附测定法（《儿科学研究》1985年；19: 354 - 357）测量了正常受试者、肝细胞癌患者以及患有其他各种疾病患者的血浆异常凝血酶原（去γ - 羧基凝血酶原；DCP）水平。52例肝细胞癌患者中有58%的患者DCP水平升高；28例晚期或中度晚期肝细胞癌患者中有24例呈阳性。相比之下，50名正常对照者、13名孕妇和10例急性肝炎患者的水平正常。55例慢性肝病患者中有3例，32例其他恶性肿瘤患者中有6例略有升高。因此，血浆DCP升高似乎有助于肝细胞癌的诊断。为了阐明肝细胞癌中DCP升高的机制，我们培养了一种人肝癌细胞系huH - 2，并测量了培养基中这种异常凝血酶原的水平。huH - 2细胞在不添加维生素K的培养基中产生大量DCP。其以细胞浓度和时间依赖性方式增加。在添加维生素K的培养基中，这些细胞在培养基中未产生可检测到的DCP量。因此，人肝癌细胞系huH - 2产生DCP，其产生取决于培养基中可用的维生素K量。去γ - 羧基凝血酶原可能是用于诊断肝细胞癌的一种有用的肿瘤标志物。
