从沙特阿拉伯骆驼家族的牛奶中纯化的乳铁蛋白的免疫原性比较。

Immunogenicity comparison of lactoferrin purified from Saudi Arabia camel clans milk.

作者信息

Almehdar Hussein A, El-Baky Nawal Abd, Alhaider Abdulqader A, Redwan Elrashdy M

机构信息

Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, New Borg EL-Arab, Alexandria, Egypt.

出版信息

Hum Antibodies. 2019;27(2):85-90. doi: 10.3233/HAB-180351.

DOI:10.3233/HAB-180351

PMID:30372672

Abstract

Secretory lactoferrins play a crucial rolls at mucosal surfaces as not only antimicrobial molecules in primate as well as human, but as physiological protein. Its multiple functions extended to be one of immunogen could elicited autoimmune disorders. Purified camel lactoferrin (cLfs) from different Saudi camel clans were shown to be a potent immunogen when injected into rabbit. Four rabbit were subcutaneously immunized with different camel clans lactoferrin/Freunds adjuvant. Anti-cLfs potency titration was reach 1:32000 and did not significantly differences between different cLfs. The cross-reactivity level of different anti-Lfs were highly significant, specially between cLfs and bLf/hLf.

摘要

分泌型乳铁蛋白在黏膜表面发挥着关键作用，它不仅是灵长类动物和人类的抗菌分子，也是一种生理蛋白。其多种功能扩展到作为一种免疫原，可能引发自身免疫性疾病。从沙特不同骆驼家族纯化得到的骆驼乳铁蛋白（cLfs），当注射到兔子体内时显示出是一种有效的免疫原。四只兔子用来自不同骆驼家族的乳铁蛋白/弗氏佐剂进行皮下免疫。抗cLfs效价滴定达到1:32000，不同cLfs之间无显著差异。不同抗Lfs的交叉反应水平非常显著，特别是cLfs与bLf/hLf之间。