薯蓣皂苷对碘酸钠诱导的骨关节炎大鼠模型软骨破坏的保护作用。
Protective effects of dioscin against cartilage destruction in a monosodium iodoacetate (MIA)-indcued osteoarthritis rat model.
机构信息
Department of Orthopaedics, First Affiliated Hospital, Dalian Medical University, Zhongshan Road No. 222, Dalian, 116011, China.
Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, 116044, China.
出版信息
Biomed Pharmacother. 2018 Dec;108:1029-1038. doi: 10.1016/j.biopha.2018.09.075. Epub 2018 Sep 28.
BACKGROUND
Osteoarthritis (OA) is a disabling disease of joint with no clear treatment. The finding of medicine be of benefit to joint is an important topic for osteoarthritis prevention and treatment. The present study was designed to explore the therapeutic effects and possible underlying mechanism of dioscin, a natural steroidal saponin, on osteoarthritis.
METHODS
OA models were created via intra-joint injection of monosodium iodoacetate (MIA) in rats. After the administration of dioscin, the effects of dioscin were estimated with western blotting, qRT-PCR and histologic staining.
RESULTS
The results showed that dioscin exerted cartilage and extracelluar matrix (ECM) protective effects via suppressing ER-stress, oxidative stress, apoptosis and inflammation. More significantly, it also ameliorated the progress of OA via inhibiting Wnt/β-catenin pathway and up-regulating PPAR-γ expression.
CONCLUSION
Our work showed the good protective effects of dioscin on MIA-induced OA for the first time. Dioscin is a promising drug on OA treatment although further researches are needed in the future.
背景
骨关节炎（OA）是一种关节功能丧失的疾病，目前尚无明确的治疗方法。寻找对关节有益的药物是 OA 防治的重要课题。本研究旨在探讨天然甾体皂甙薯蓣皂甙对骨关节炎的治疗作用及可能的作用机制。
方法
通过向大鼠关节内注射单碘乙酸盐（MIA）建立 OA 模型。薯蓣皂甙给药后，通过 Western blot、qRT-PCR 和组织学染色评估薯蓣皂甙的作用。
结果
结果表明，薯蓣皂甙通过抑制内质网应激、氧化应激、细胞凋亡和炎症，发挥软骨和细胞外基质（ECM）保护作用。更重要的是，它还通过抑制 Wnt/β-catenin 通路和上调 PPAR-γ 表达，改善 OA 的进展。
结论
本研究首次表明薯蓣皂甙对 MIA 诱导的 OA 具有良好的保护作用。尽管未来还需要进一步研究，但薯蓣皂甙是一种有前途的 OA 治疗药物。
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