高压氧对全脑缺血再灌注损伤大鼠血脑屏障通透性的影响。
Effect of hyperbaric oxygen on the permeability of the blood-brain barrier in rats with global cerebral ischemia/reperfusion injury.
机构信息
Center of Emergency & Intensive Care Unit, Shanghai, 201508, PR China; Medical Center of Chemical Injury, Shanghai, 201508, PR China; Medical Research Centre for Chemical Injury, Emergency and Critical Care, Jinshan Hospital, Fudan University, Shanghai, 201508, PR China.
Center of Emergency & Intensive Care Unit, Shanghai, 201508, PR China; Medical Center of Chemical Injury, Shanghai, 201508, PR China; Medical Research Centre for Chemical Injury, Emergency and Critical Care, Jinshan Hospital, Fudan University, Shanghai, 201508, PR China.
出版信息
Biomed Pharmacother. 2018 Dec;108:1725-1730. doi: 10.1016/j.biopha.2018.10.025. Epub 2018 Oct 12.
OBJECTIVE
The aim of this study was to investigate the effects of hyperbaric oxygen on the permeability of the blood-brain barrier in rats with global cerebral ischemia/reperfusion injury and explore possible mechanisms.
METHODS
A rat model of global cerebral ischemia/reperfusion injury established via Pulsinelli four-vessel occlusion method and a total of 162 Wistar rats were randomly divided into three groups, including sham group, global cerebral ischemia/reperfusion group (IR group) and hyperbaric oxygen treated group (HBO group). Permeability of the blood-brain barrier of these rats were evaluated by Evans Blue staining. The expression of caveolin-1 and tight junction protein ZO-1 was examined by Immunohistochemistry staining and western-blotting.
RESULTS
Successfully establishment of the rat model was verified by W:D ratio, and significantly increased Evans Blue level was found in IR group compared to control group, whereas hyperbaric treatment could result in decreased Evans Blue level in HBO group. Increased expression of caveolin-1 and tight junction protein ZO-1 were found in rats with hyperbaric oxygen exposure compared to those in IS group.
CONCLUSIONS
Hyperbaric oxygen exposure improved the permeability of the blood-brain barrier in rats with global cerebral ischemia/reperfusion injury, and increased expression of caveolin-1 and tight junction protein ZO-1 were involved in the mechanisms.
目的
本研究旨在探讨高压氧对全脑缺血再灌注损伤大鼠血脑屏障通透性的影响，并探讨可能的机制。
方法
采用 Pulsinelli 四血管闭塞法建立大鼠全脑缺血再灌注损伤模型，将 162 只 Wistar 大鼠随机分为假手术组、全脑缺血再灌注组（IR 组）和高压氧治疗组（HBO 组）。通过伊文思蓝染色评估大鼠血脑屏障通透性。免疫组织化学染色和 Western blot 检测 caveolin-1 和紧密连接蛋白 ZO-1 的表达。
结果
通过 W:D 比值验证了大鼠模型的成功建立，与对照组相比，IR 组的伊文思蓝水平明显升高，而高压氧治疗可使 HBO 组的伊文思蓝水平降低。与 IS 组相比，高压氧暴露组大鼠 caveolin-1 和紧密连接蛋白 ZO-1 的表达增加。
结论
高压氧暴露可改善全脑缺血再灌注损伤大鼠血脑屏障通透性，增加 caveolin-1 和紧密连接蛋白 ZO-1 的表达可能参与了这一机制。
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