细胞膜上的配体-受体结合：动态力谱学应用

Ligand-Receptor Binding on Cell Membrane: Dynamic Force Spectroscopy Applications.

作者信息

Liu Jianli, Li Wenhui, Zhang Xuejie, Feng Yan, Fang Xiaohong

机构信息

Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Methods Mol Biol. 2019;1886:153-162. doi: 10.1007/978-1-4939-8894-5_8.

DOI:10.1007/978-1-4939-8894-5_8

PMID:30374866

Abstract

Ligand-receptor recognition on the cell membrane enables the communication of cells with the extracellular environment. Atomic force microscopy (AFM)-based single-molecule dynamic force spectroscopy represents one of the most powerful techniques available to directly investigate ligand-receptor recognition under physiological conditions without considerable disruption to cells. It provides important information for research on biological processes, disease pathogenesis, and mechanism of drugs. Here we describe an example of applying single-molecule dynamic force spectroscopy to study the binding of epidermal growth factor (EGF) to its receptor EGFR, as well as the effect of two clinical drugs, Pertuzumab and Trastuzumab, on the interaction of EGF and EGFR.

摘要

细胞膜上的配体-受体识别使细胞能够与细胞外环境进行通讯。基于原子力显微镜（AFM）的单分子动态力谱是直接研究生理条件下配体-受体识别且对细胞干扰较小的最强大技术之一。它为生物过程、疾病发病机制和药物作用机制的研究提供了重要信息。在此，我们描述了一个应用单分子动态力谱研究表皮生长因子（EGF）与其受体表皮生长因子受体（EGFR）结合的例子，以及两种临床药物帕妥珠单抗和曲妥珠单抗对EGF与EGFR相互作用的影响。