Eghiaian Frédéric, Rico Felix, Colom Adai, Casuso Ignacio, Scheuring Simon
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France.
U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France.
FEBS Lett. 2014 Oct 1;588(19):3631-8. doi: 10.1016/j.febslet.2014.06.028. Epub 2014 Jun 14.
Atomic force microscopy (AFM) is the type of scanning probe microscopy that is probably best adapted for imaging biological samples in physiological conditions with submolecular lateral and vertical resolution. In addition, AFM is a method of choice to study the mechanical unfolding of proteins or for cellular force spectroscopy. In spite of 28 years of successful use in biological sciences, AFM is far from enjoying the same popularity as electron and fluorescence microscopy. The advent of high-speed atomic force microscopy (HS-AFM), about 10 years ago, has provided unprecedented insights into the dynamics of membrane proteins and molecular machines from the single-molecule to the cellular level. HS-AFM imaging at nanometer-resolution and sub-second frame rate may open novel research fields depicting dynamic events at the single bio-molecule level. As such, HS-AFM is complementary to other structural and cellular biology techniques, and hopefully will gain acceptance from researchers from various fields. In this review we describe some of the most recent reports of dynamic bio-molecular imaging by HS-AFM, as well as the advent of high-speed force spectroscopy (HS-FS) for single protein unfolding.
原子力显微镜（AFM）是扫描探针显微镜的一种类型，可能最适合在生理条件下对生物样品进行成像，具有亚分子级的横向和纵向分辨率。此外，AFM是研究蛋白质机械解折叠或进行细胞力谱分析的首选方法。尽管AFM在生物科学领域已成功应用了28年，但它远未像电子显微镜和荧光显微镜那样广受欢迎。大约10年前高速原子力显微镜（HS-AFM）的出现，为从单分子水平到细胞水平的膜蛋白和分子机器动力学提供了前所未有的见解。纳米分辨率和亚秒级帧率的HS-AFM成像可能会开辟新的研究领域，描绘单生物分子水平的动态事件。因此，HS-AFM是其他结构和细胞生物学技术的补充，有望获得各个领域研究人员的认可。在这篇综述中，我们描述了一些关于HS-AFM动态生物分子成像的最新报道，以及用于单蛋白解折叠的高速力谱（HS-FS）的出现。
