Los Alamos National Laboratory, Los Alamos, NM, USA.
New Mexico Consortium, Los Alamos, NM, USA.
Nat Methods. 2018 Nov;15(11):905-908. doi: 10.1038/s41592-018-0173-1. Epub 2018 Oct 30.
We report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix.map_to_model. We applied our approach to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at resolutions worse than 3 Å.
我们报告了一种完全自动化的程序，用于优化和解释低温电子显微镜（cryo-EM）数据的重建，该程序可在 Phenix 中作为 phenix.map_to_model 使用。我们将该方法应用于 476 个分辨率为 4.5 Å 或更好的数据集，包括 47 个核糖体和 32 个其他蛋白质-RNA 复合物的重建。在分辨率为 3 Å 或更好的重建中，自动重现的残基中位数为 71%，在分辨率差于 3 Å 的重建中为 47%。
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