在中国北方，慢性鼻-鼻窦炎伴鼻息肉患者鼻黏膜中调节粘蛋白型 O-聚糖生物合成和转化生长因子 β 信号通路的 microRNAs。
MicroRNAs regulating mucin type O-glycan biosynthesis and transforming growth factor β signaling pathways in nasal mucosa of patients with chronic rhinosinusitis with nasal polyps in Northern China.
机构信息
Department of Otorhinolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China.
Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
出版信息
Int Forum Allergy Rhinol. 2019 Jan;9(1):106-113. doi: 10.1002/alr.22230. Epub 2018 Oct 31.
BACKGROUND
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent sinonasal mucosa inflammatory disease. MicroRNAs (miRNAs) that are involved in the pathogenesis of CRSwNP in Northern China remain unknown.
METHODS
A miRCURY™ LNA Array was used to analyze miRNA profiles in nasal mucosa tissues of CRSwNP patients (n = 19) and healthy controls (n = 10). Subsequent pathways were predicted by DIANA-mirPath software.
RESULTS
Five upregulated miRNAs, including miR-210-5p, miR-3178, miR-585-3p, miR-3146, and miR-320e, and 19 downregulated miRNAs, including miR-32-3p, miR-1299, miR-3196, miR-3924, and miR-548e-3p, were differentially expressed (p < 0.05, fold change >2) in tissues of CRSwNP vs controls. Utilizing the Kyoto Encyclopedia of Genes and Genomes database (KEGG), which is an online database for pathway mapping, mucin type O-glycan biosynthesis pathway was significantly enriched in upregulated miRNAs. Transforming growth factor-beta (TGF-β), transient receptor potential (TRP) channels, and the mitogen-activated protein kinase (MAPK) signaling pathway were significantly linked to downregulated miRNAs.
CONCLUSION
The mucin type O-glycan biosynthesis pathway and TGF-β signaling pathway are regulated by miRNAs, which could be our focus in the future studies.
背景
伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）是一种持续性的鼻窦黏膜炎症性疾病。在中国北方，参与 CRSwNP 发病机制的 microRNAs（miRNAs）尚不清楚。
方法
使用 miRCURY™ LNA Array 分析了 19 例 CRSwNP 患者（n=19）和 10 例健康对照者（n=10）的鼻黏膜组织中的 miRNA 图谱。随后使用 DIANA-mirPath 软件预测了相关通路。
结果
与对照组相比，CRSwNP 组织中存在 5 个上调 miRNA（包括 miR-210-5p、miR-3178、miR-585-3p、miR-3146 和 miR-320e）和 19 个下调 miRNA（包括 miR-32-3p、miR-1299、miR-3196、miR-3924 和 miR-548e-3p），差异具有统计学意义（p＜0.05，倍数变化＞2）。利用京都基因与基因组百科全书（KEGG）数据库（KEGG 是一个用于通路映射的在线数据库）对差异表达 miRNA 进行通路分析，发现粘蛋白型 O-聚糖生物合成途径显著富集。转化生长因子-β（TGF-β）、瞬时受体电位（TRP）通道和丝裂原活化蛋白激酶（MAPK）信号通路与下调的 miRNA 显著相关。
结论
miRNAs 调控粘蛋白型 O-聚糖生物合成途径和 TGF-β 信号通路，这可能是我们未来研究的重点。
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