Wu Zhenning, Xue Shujiang, Yang Yongjie
Agricultural College of Yanbian University, Yanji 133002, China.
Se Pu. 2018 Oct 8;36(10):947-951. doi: 10.3724/SP.J.1123.2018.05019.
Nucleic acid aptamers are single-stranded oligonucleotides that possess high affinity and specificity. They are also known as "chemical antibodies" and have a wide range of applications. Aptamers are usually generated by an in vitro process termed as systematic evolution of ligands by exponential enrichment (SELEX). Aptamers are often selected by employing purified soluble protein targets. However, the process of protein purification can be complex and laborious. Furthermore, several protein targets, such as low abundance proteins found in serums and membrane proteins found in cells, are difficult to purify. Complex target SELEX can avoid the purification step and maintain the native state of the target proteins. Even in the absence of detailed composition and characterization of a complex target, complex target SELEX can be performed with a high throughput for obtaining specific aptamers. In this study, complex target SELEX taken unpurified biological samples as targets will be described in order to provide a new idea for researchers screening aptamers.
核酸适体是具有高亲和力和特异性的单链寡核苷酸。它们也被称为“化学抗体”，具有广泛的应用。适体通常通过一种称为指数富集配体系统进化（SELEX）的体外过程产生。适体通常通过使用纯化的可溶性蛋白质靶标来筛选。然而，蛋白质纯化过程可能复杂且费力。此外，一些蛋白质靶标，如血清中发现的低丰度蛋白质和细胞中发现的膜蛋白，很难纯化。复杂靶标SELEX可以避免纯化步骤并保持靶标蛋白的天然状态。即使在没有复杂靶标详细组成和表征的情况下，复杂靶标SELEX也可以高通量进行以获得特异性适体。在本研究中，将描述以未纯化的生物样品为靶标的复杂靶标SELEX，以便为研究人员筛选适体提供新思路。
