Shibata H, Ogata E, Etoh Y, Shibai H, Kojima I
Biochem Biophys Res Commun. 1987 Jul 15;146(1):187-93. doi: 10.1016/0006-291x(87)90709-1.
We examined an early action of erythroid differentiation factor (EDF), a polypeptide which induces differentiation of Friend murine erythroleukemia (MEL) cells. (Eto et al., Biochem. Biophys. Res. Commun. 142: 1095-1103, 1987). In MEL cells, EDF caused a rapid and transient increase in cytoplasmic concentration of free calcium, [Ca2+]c. EDF increased [Ca2+]c even in the absence of extracellular calcium. When [3H]inositol-labeled MEL cells were incubated with EDF, EDF rapidly increased radioactivity in inositol trisphosphate, bisphosphate and monophosphate. EDF also increased [3H] diacylglycerol in [3H]arachidonate-labeled MEL cells. These results indicate that EDF increases [Ca2+]c by stimulating hydrolysis of polyphosphoinositide.
我们研究了红细胞分化因子（EDF）的早期作用，EDF是一种能诱导弗氏小鼠红白血病（MEL）细胞分化的多肽。（Eto等人，《生物化学与生物物理研究通讯》142: 1095 - 1103, 1987）。在MEL细胞中，EDF使游离钙的细胞质浓度[Ca2+]c迅速且短暂地升高。即使在没有细胞外钙的情况下，EDF也能提高[Ca2+]c。当用[3H]肌醇标记的MEL细胞与EDF一起孵育时，EDF迅速增加了三磷酸肌醇、二磷酸肌醇和单磷酸肌醇中的放射性。EDF还增加了用[3H]花生四烯酸标记的MEL细胞中的[3H]二酰甘油。这些结果表明，EDF通过刺激多磷酸肌醇的水解来提高[Ca2+]c。
