Department of Biofunctional Informatics, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
Department of Clinical Laboratory, Medical Hospital, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
Sci Rep. 2018 Oct 31;8(1):16129. doi: 10.1038/s41598-018-34229-6.
An easy-to-use assessment for activated factor X (FXa) is lacking despite its pivotal role in the coagulation. Dielectric blood coagulometry (DBCM) was recently invented as a novel assessment tool for determining the whole blood coagulability by measuring the temporal change in the permittivity of blood. We previously reported that it could evaluate the global blood coagulability. This study aimed to apply the DBCM for assessing FXa activity and its inhibition by anticoagulants. We performed the DBCM analysis along with measurement of the FXa activity by a fluorometric assay in samples from healthy subjects, and identified a new index named maximum acceleration time (MAT) that had a correlation to the FXa activity. Next the DBCM analysis was performed using blood samples mixed with anticoagulants (unfractionated heparin, dalteparin, and edoxaban). Blood samples with three anticoagulants had different profiles of the temporal change in the permittivity, reflecting their different selectivity for FXa. We compared the MAT with the anti-FXa activity assay, and found that the prolongation of MAT was similarly correlated with the anti-FXa activity regardless of the type of anticoagulants. In conclusion, the DBCM has the possibility for evaluating the innate FXa activity and effect of anticoagulants focusing on their FXa inhibition.
尽管激活的因子 X（FXa）在凝血中起着关键作用，但目前仍缺乏一种易于使用的 FXa 评估方法。介电血液凝固测定法（DBCM）是最近发明的一种新的评估工具，通过测量血液介电常数的时间变化来确定全血的可凝固性。我们之前曾报道过它可以评估整体血液凝固性。本研究旨在应用 DBCM 评估 FXa 活性及其被抗凝剂抑制的情况。我们对健康受试者的样本同时进行 DBCM 分析和 FXa 活性的荧光测定，并确定了一个新的指标，称为最大加速度时间（MAT），与 FXa 活性相关。接下来，我们使用抗凝剂（未分级肝素、达肝素和依度沙班）混合的血液样本进行 DBCM 分析。三种抗凝剂的血液样本具有不同的介电常数时间变化曲线，反映了它们对 FXa 的选择性不同。我们将 MAT 与抗 FXa 活性测定进行了比较，发现无论抗凝剂的类型如何，MAT 的延长与抗 FXa 活性均具有相似的相关性。总之，DBCM 有可能评估内在的 FXa 活性和抗凝剂的作用，重点是它们对 FXa 的抑制作用。
