Roberts B L, Richardson W D, Smith A E
Cell. 1987 Jul 31;50(3):465-75. doi: 10.1016/0092-8674(87)90500-9.
The effect of position and number and of another intracellular location signal on the activity of the nuclear location signal was investigated. A minimal signal was inserted into several sites within the polypeptide chain of pyruvate kinase. The results observed suggest that a nuclear location signal can function at a variety of positions within a protein but that in some locations its activity is masked. Multiple copies of a partially defective signal were integrated into pyruvate kinase. The data suggest that multiple signals can cooperate to enhance nuclear accumulation. A nuclear location signal failed to function when inserted into polyomavirus middle T but was active in an identical variant lacking the carboxy-terminal hydrophobic tail. We conclude that while a minimal nuclear location signal is sufficient for nuclear localization, its activity is crucially dependent on the protein context within which it is present.
研究了位置、数量以及另一种细胞内定位信号对核定位信号活性的影响。将一个最小信号插入丙酮酸激酶多肽链内的几个位点。观察到的结果表明，核定位信号可以在蛋白质内的多种位置发挥作用，但在某些位置其活性会被掩盖。将部分缺陷信号的多个拷贝整合到丙酮酸激酶中。数据表明多个信号可以协同作用以增强核积累。当插入多瘤病毒中间T抗原时，核定位信号不起作用，但在缺乏羧基末端疏水尾巴的相同变体中具有活性。我们得出结论，虽然最小的核定位信号足以实现核定位，但其活性关键取决于它所处的蛋白质环境。
