Morris B J, Taylor J E
Clin Exp Pharmacol Physiol. 1987 Jan;14(1):23-31. doi: 10.1111/j.1440-1681.1987.tb00953.x.
Inactive renin has been studied extensively in human plasma, but in animal plasma its accurate quantification has proved more difficult, due partly to higher activity of plasma protease inhibitors. Such activity in human plasma can be conveniently destroyed by a metalloprotease in Bitis arietans venom, with concommitant release of endogenous enzyme activities, such as plasma kallikrein, that then activate inactive renin. It was therefore of interest to look for inactive renin in rat and rabbit plasma using this approach, so providing, in addition, a comparison for the disparate data of other groups who have used trypsin or acid for activation. In both rat and rabbit plasma the proportion of inactive renin was 62% of total renin, whereas human plasma contained more inactive renin and a higher proportion, 82%. A higher concentration of venom was required for rat (33 ug venom/ml plasma) and rabbit (4 micrograms/ml) than needed for activation, at a similar rate, in human plasma (1 microgram/ml). When applied to studies of rats made hypertensive and hyper-reninaemic by aortic ligation for 5 days, higher total (active + inactive) renin was observed. The proportion of inactive renin, as a percentage of total renin in plasma collected at this time, was, however, found to diminish significantly. In conclusion, puff adder venom activates inactive renin in rat and rabbit plasma and can be used to study physiological changes in inactive renin in such animal plasma.
无活性肾素已在人血浆中得到广泛研究，但在动物血浆中，其准确定量已被证明更困难，部分原因是血浆蛋白酶抑制剂的活性较高。人血浆中的这种活性可以通过膨蝰毒液中的一种金属蛋白酶方便地破坏，同时释放内源性酶活性，如血浆激肽释放酶，进而激活无活性肾素。因此，利用这种方法在大鼠和兔血浆中寻找无活性肾素很有意义，此外，还能为其他使用胰蛋白酶或酸进行激活的研究小组的不同数据提供比较。在大鼠和兔血浆中，无活性肾素占总肾素的比例均为62%，而人血浆中无活性肾素含量更多，比例更高，为82%。与在人血浆（1微克/毫升）中以相似速率激活所需的毒液浓度相比，大鼠（33微克毒液/毫升血浆）和兔（4微克/毫升）需要更高浓度的毒液。当应用于对通过主动脉结扎5天而导致高血压和高肾素血症的大鼠的研究时，观察到总（活性+无活性）肾素更高。然而，此时采集的血浆中，无活性肾素占总肾素的百分比显著降低。总之，膨蝰毒液可激活大鼠和兔血浆中的无活性肾素，并可用于研究此类动物血浆中无活性肾素的生理变化。
