McAuliffe Gary N, Taylor Susan L, Moore Sharon, Hewitt Joanne, Upton Arlo, Howe Anna S, Best Emma J
Microbiology Department, Labtests and Northland Pathology Laboratories, Auckland, New Zealand.
Microbiology Department, Middlemore Hospital, Auckland, New Zealand.
Diagn Microbiol Infect Dis. 2019 Mar;93(3):203-207. doi: 10.1016/j.diagmicrobio.2018.10.003. Epub 2018 Oct 11.
Rotavirus vaccine has reduced disease prevalence in many countries. Consequently, we aimed to assess the reliability of a rotavirus immunoassay in the community population of Auckland and Northland, New Zealand. Between 22 October 2015 and 31 December 2016, 2873 fecal samples were tested by enzyme immunoassay (EIA, Rotascreen II, Microgen, UK) from 2748 patients (median age 8 years, range 0-101 years). Eighty-nine (3.1%) samples were reactive; 86 samples were tested by a second method. Rotavirus was confirmed in 49/86 (57%). Positive rotavirus EIAs were more likely to be confirmed in samples from cases ≥1 year of age (positive predictive value [PPV] 61%, 95% confidence interval [CI] 50-72%, P = 0.049) and in spring/summer (PPV 67%, 95% CI 55-78%, P = 0.003). Reactive rotavirus tests required confirmatory testing regardless of demographic, vaccine, or seasonal factors; a review of rotavirus testing algorithms may be necessary in other vaccinated community populations.
轮状病毒疫苗已在许多国家降低了疾病的流行率。因此，我们旨在评估一种轮状病毒免疫测定法在新西兰奥克兰和北地社区人群中的可靠性。在2015年10月22日至2016年12月31日期间，对来自2748名患者（年龄中位数8岁，范围0 - 101岁）的2873份粪便样本进行了酶免疫测定（EIA，Rotascreen II，Microgen，英国）检测。八十九份（3.1%）样本呈反应性；86份样本采用第二种方法进行了检测。49/86（57%）的样本确诊为轮状病毒感染。年龄≥1岁的病例样本中轮状病毒EIA检测呈阳性更有可能得到确诊（阳性预测值[PPV] 61%，95%置信区间[CI] 50 - 72%，P = 0.049），且在春夏季也是如此（PPV 67%，95% CI 55 - 78%，P = 0.003）。无论人口统计学、疫苗或季节因素如何，轮状病毒检测呈反应性均需要进行确诊检测；在其他已接种疫苗的社区人群中可能有必要对轮状病毒检测算法进行审查。
