Department of Radiology, The First Affiliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei 230022, China.
Department of Nuclear medicine, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
Acad Radiol. 2019 Aug;26(8):e224-e232. doi: 10.1016/j.acra.2018.09.011. Epub 2018 Oct 30.
To investigate the feasibility of determining quantitative parameters for evaluating tumor hypoxia in the C6 glioma model by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).
Thirty male Sprague-Dawley rats were inoculated to establish C6 brain glioma models. DCE-MRI scans were performed 14, 21, and 28 days after transplantation. Quantitative parameters comprising K, V, K, and V were calculated and analyzed. At the end of each scan, 10 rats were randomly selected for immunohistochemical (IHC) staining of hypoxia-inducible factor-17αl (HIF-1α), proliferating cell nuclear antigen (PCNA), and CD34. Correlations between quantitative parameters and IHC scores were analyzed.
The tumor volumes increased with time. The K values on days 14, 21, and 28 were 0.03 ± 0.009 min, 0.084 ± 0.010 min, and 0.050 ± 0.016 min, while the V values were 0.17 ± 0.070, 0.46 ± 0.159, and 0.51 ± 0.193, the K values were 0.18 ± 0.070%, 0.220 ± 0.049%, and 0.06 ± 0.035%, and these three parameters all differed significantly among the three time points. The V values on days 14, 21, and 28 were 0.09 ± 0.040%, 0.120 ± 0.034%, and 0.06 ± 0.010%, but the values did not differ among the three time points (P = 0.073). K had significant negative correlations with the HIF-1α scores on days 14 and 21 when there was also a positive correlation between K and CD34. V had negative correlations with the HIF-1α score on days 14 and 21, and there was a negative correlation between V and PCNA on day 21. K had a negative correlation with the HIF-1α score and a positive correlation with MVD on day 21.
DCE-MRI may be a useful method for the noninvasive evaluation of the hypoxia status in a glioma model.
通过动态对比增强磁共振成像（DCE-MRI）研究定量参数评估 C6 神经胶质瘤模型肿瘤缺氧的可行性。
30 只雄性 Sprague-Dawley 大鼠接种建立 C6 脑胶质瘤模型。移植后 14、21 和 28 天进行 DCE-MRI 扫描。计算并分析包括 K、V、K 和 V 的定量参数。每次扫描结束时，随机选择 10 只大鼠进行缺氧诱导因子-1α（HIF-1α）、增殖细胞核抗原（PCNA）和 CD34 的免疫组织化学（IHC）染色。分析定量参数与 IHC 评分之间的相关性。
肿瘤体积随时间而增加。第 14、21 和 28 天的 K 值分别为 0.03 ± 0.009 min、0.084 ± 0.010 min 和 0.050 ± 0.016 min，V 值分别为 0.17 ± 0.070、0.46 ± 0.159 和 0.51 ± 0.193，K 值分别为 0.18 ± 0.070%、0.220 ± 0.049% 和 0.06 ± 0.035%，这三个参数在三个时间点之间均有显著差异。第 14、21 和 28 天的 V 值分别为 0.09 ± 0.040、0.120 ± 0.034 和 0.06 ± 0.010%，但三个时间点之间的 V 值无显著差异（P = 0.073）。K 值与第 14 天和第 21 天的 HIF-1α 评分呈显著负相关，同时 K 值与 CD34 呈正相关。V 值与第 14 天和第 21 天的 HIF-1α 评分呈负相关，第 21 天的 V 值与 PCNA 呈负相关。K 值与 HIF-1α 评分呈负相关，与第 21 天的 MVD 呈正相关。
DCE-MRI 可能是评估胶质瘤模型缺氧状态的一种有用的非侵入性方法。
