Department of Clinical Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, No. 20, Yuhuangding East Road, Yantai 264000, Shandong Province, PR China.
Department of Clinical Laboratory, Yantaishan Hospital, Yantai 264001, Shandong Province, PR China.
Clin Res Hepatol Gastroenterol. 2019 Jun;43(3):282-291. doi: 10.1016/j.clinre.2018.09.011. Epub 2018 Oct 29.
Hepatocellular carcinoma (HCC) is a complex and heterogeneous tumor with several genomic alterations, while the viral-chemical etiology along with molecular mechanisms of HCC pathogenesis remains largely unknown. This study aimed to determine expression profile and prognostic value of HER-2/neu, STAT3 and SOCS3 in HCC.
Immunohistochemistry and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were performed to evaluate the expression of HER-2/neu, STAT3 and SOCS3 in HCC tissues and adjacent normal tissues collected from 176 HCC patients.
HER-2/neu and STAT3 levels were higher and SOCS3 expression was lower in HCC tissues than in adjacent normal tissues. HER-2/neu, STAT3 and SOCS3 levels were associated with histological grade, tumor diameter, TNM stage, vascular invasion, lymph node metastasis and distant metastasis in HCC. SOCS3 expression was negatively associated with HER-2/neu and STAT3 expression. HCC patients with higher HER-2/neu and STAT3 levels had shorter overall, disease-free and disease-specific survival, whereas the opposite was found in patients with higher SOCS3 expression. In Cox regression analysis, tumor size, TNM stage, and STAT3 expression were identified as independent prognostic factors of HCC.
Taken together, these observations suggest that HER-2/neu, STAT3 and, SOCS3 are related to the aggressive tumor behavior and STAT3 has potential value as a prognostic factor for HCC.
肝细胞癌（HCC）是一种具有多种基因组改变的复杂异质性肿瘤，而 HCC 的发病机制的病毒-化学病因和分子机制在很大程度上仍然未知。本研究旨在确定 HER-2/neu、STAT3 和 SOCS3 在 HCC 中的表达谱和预后价值。
采用免疫组织化学和逆转录定量聚合酶链反应（RT-qPCR）检测 176 例 HCC 患者的 HCC 组织和相邻正常组织中 HER-2/neu、STAT3 和 SOCS3 的表达。
与相邻正常组织相比，HER-2/neu 和 STAT3 水平在 HCC 组织中较高，SOCS3 表达较低。HER-2/neu、STAT3 和 SOCS3 水平与 HCC 的组织学分级、肿瘤直径、TNM 分期、血管侵犯、淋巴结转移和远处转移有关。SOCS3 表达与 HER-2/neu 和 STAT3 表达呈负相关。HER-2/neu 和 STAT3 水平较高的 HCC 患者总生存期、无病生存期和疾病特异性生存期较短，而 SOCS3 表达较高的患者则相反。在 Cox 回归分析中，肿瘤大小、TNM 分期和 STAT3 表达被确定为 HCC 的独立预后因素。
综上所述，这些观察结果表明，HER-2/neu、STAT3 和 SOCS3 与侵袭性肿瘤行为有关，STAT3 作为 HCC 的预后因素具有潜在价值。
