MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Gene. 2019 Feb 15;685:114-124. doi: 10.1016/j.gene.2018.10.081. Epub 2018 Oct 30.
Sucrose synthase and sucrose transporter are involved in sucrose metabolism and partitioning of photosynthetic products, respectively. In this study, we cloned SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 genes from ramie. Real-time quantitative PCR revealed that BnSUS1 and BnSUT2 were widely expressed in the analyzed tissues. Subsequently, the two promoters of BnSUS1 and BnSUT2 were isolated and truncated. The two promoters and their truncated fragments were fused GUS to transform into Arabidopsis. GUS staining showed that BnSUS1-1690 and BnSUS1-1420 had vascular specificity in cotyledons and mature leaves while BnSUT2-2239, BnSUT2-1681, BnSUT2-1199 and BnSUT2-618 had a constitutive function in seedlings and mature organs. Notably, the activity of BnSUT2-2239 and its fragments (except that of BnSUT2-231) are strongly induced by mechanical wounding. Moreover, BnSUS1-1051 and BnSUS1-485 are sensitive to CuSO treatment while BnSUT2-2239 and BnSUT2-1681 are sensitive to PEG and ABA treatments, respectively. Our findings will provide the foundation for deciphering the functions of BnSUS1 and BnSUT2, and also expand the promoter library to provide more options for plant genetic engineering.
蔗糖合酶和蔗糖转运蛋白分别参与蔗糖代谢和光合产物的分配。本研究从苎麻中克隆了蔗糖合酶 1 和蔗糖转运蛋白 2 基因。实时定量 PCR 显示，BnSUS1 和 BnSUT2 在分析的组织中广泛表达。随后，分离并截短了 BnSUS1 和 BnSUT2 的两个启动子。将这两个启动子及其截短片段与 GUS 融合并转化拟南芥。GUS 染色显示，BnSUS1-1690 和 BnSUS1-1420 在子叶和成熟叶片中有血管特异性，而 BnSUT2-2239、BnSUT2-1681、BnSUT2-1199 和 BnSUT2-618 在幼苗和成熟器官中具有组成型功能。值得注意的是，BnSUT2-2239 及其片段（BnSUT2-231 除外）的活性被机械损伤强烈诱导。此外，BnSUS1-1051 和 BnSUS1-485 对 CuSO 处理敏感，而 BnSUT2-2239 和 BnSUT2-1681 分别对 PEG 和 ABA 处理敏感。我们的研究结果将为解析 BnSUS1 和 BnSUT2 的功能提供基础，并为植物遗传工程提供更多的启动子库选择。
