蛙类视杆细胞外段中钙依赖性环鸟苷酸结合蛋白的检测

Detection of Ca2+-dependent cyclic GMP binding protein in frog rod outer segments.

作者信息

Shinozawa T, Terada S, Matsusaka H, Yamashita S

出版信息

FEBS Lett. 1987 Jul 27;219(2):293-5. doi: 10.1016/0014-5793(87)80238-7.

Abstract

For the identification of the cGMP-sensitive ion channel protein of frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by photoaffinity labeling with [3H]cGMP. We found three cGMP binding polypeptides (66 kDa, 92 kDa and 100 kDa) in the membrane protein fraction of ROS. cGMP binding to the 66 kDa polypeptide required the addition of 2 mM CaCl2. We propose that this polypeptide corresponds to the cGMP-activated channel protein reported by Cook et al. [(1987) Proc. Natl. Acad. Sci. USA 84, 585-589]. The 100 kDa and 92 kDa polypeptides are subunits of the cGMP phosphodiesterase.

摘要

为了鉴定青蛙视杆细胞外段（ROS）的cGMP敏感离子通道蛋白，我们通过用[3H]cGMP进行光亲和标记分析了ROS中的cGMP结合蛋白。我们在ROS的膜蛋白组分中发现了三种cGMP结合多肽（66 kDa、92 kDa和100 kDa）。cGMP与66 kDa多肽的结合需要添加2 mM CaCl2。我们认为该多肽对应于Cook等人[（1987年）美国国家科学院院刊84，585 - 589]报道的cGMP激活通道蛋白。100 kDa和92 kDa多肽是cGMP磷酸二酯酶的亚基。