Tilgner S, Stelzner A, Dietze U, Hempel E, Meyer W, Schröder K D, Sych F J
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1977 Oct 28;204(2):113-23. doi: 10.1007/BF00414712.
The authors studied the appearance and behavior of complement-fixing anti-ROS antibodies in the sera of GrCh rabbits following one or two intracutaneous injections of retinal antigens emulsified in Freund's complete adjuvant (KFA). Fourteen different antigenic preparations from heterologous, homologous, and autologous retinal components incorporated in KFA were tested and compared. Apart from the supernatant of a centrifuged homogenized preparation of homologous retina, all immunogens generally induced anti-ROS antibodies. The antibody titers (reciprocal values) reached a maximum 4 to 8 weeks post injection, at only low levels. The changes in antibody concentrations showed some relationship to the quality and dose of the immunogens. Anamnestic effects following reinjection reached maximum values within as little as 5 to 14 days. Animals not developing chorioretinitic lesions showed higher antibody titers more frequently than rabbits with clinically confirmed disease. Provided there is sufficient relevance of the humoral reactions observed, the complement-fixing antibodies we found in our experiments are more likely to have protective than pathogenic functions.
作者研究了在弗氏完全佐剂（KFA）中乳化的视网膜抗原进行一次或两次皮内注射后，GrCh兔血清中补体结合抗视网膜氧化应激（ROS）抗体的出现情况和行为。测试并比较了14种来自异源、同源和自体视网膜成分并掺入KFA的不同抗原制剂。除了同源视网膜离心匀浆制剂的上清液外，所有免疫原通常都能诱导抗ROS抗体。抗体滴度（倒数）在注射后4至8周达到最高，但仅处于低水平。抗体浓度的变化与免疫原的质量和剂量有一定关系。再次注射后的回忆反应在短短5至14天内达到最大值。未发生脉络膜视网膜炎病变的动物比临床确诊疾病的兔子更频繁地出现较高的抗体滴度。假设观察到的体液反应有足够的相关性，我们在实验中发现的补体结合抗体更可能具有保护作用而非致病作用。
